National University of Cuyo, Faculty of Natural and Exact Sciences, Interdisciplinary Institute of Basic Sciences (ICB), UNCUYO-CONICET, Padre J. Contreras 1300, 5500 Mendoza, Argentina.
Universidad Nacional de Río Cuarto-CONICET, Departamento de Biología Molecular, FCEFQyN, 5800 Córdoba, Argentina.
J AOAC Int. 2023 Nov 2;106(6):1542-1549. doi: 10.1093/jaoacint/qsad073.
Fruit juices are one of the most non-alcoholic beverages consumed in the world. Essential elements and other nutrients present in fruit juices play an important role in human well-being. However, fruit juices may also contain potentially toxic elements at trace levels, causing health risks.
The objective of this work was to develop an analytical methodology based on the preconcentration of lead using a new biodegradable hybrid material (BHM) composed of Rhodococcus erythropolis AW3 bacteria and Brassica napus hairy roots.
The BHM was implemented in an online solid-phase extraction (SPE) system for the determination of lead in fruit juices by electrothermal atomic absorption spectrometry (ETAAS).
Effects of critical parameters on lead retention were studied. Under optimal experimental conditions, extraction efficiency higher than 99.9% and an enrichment factor of 62.5 were achieved. The dynamic capacity of the BHM was 36 mg/g, which favored the reuse of the column for at least eight biosorption-desorption cycles. The LOD and LOQ for preconcentration of 5 mL of sample were 5.0 and 16.5 ng/L lead, respectively. The RSD was 4.8% (at 1 µg/L lead and n = 10).
The developed method was suitable for application to lead determination in different types of fruit juice.
A novel microextraction procedure based on the use of a biohybrid adsorbent. Highly sensitive determination of Pb at trace levels. Analysis of Pb in fruit juices samples. An eco-friendly microextraction technique for Pb determination.
果汁是世界上最受欢迎的非酒精饮料之一。果汁中存在的必需元素和其他营养物质对人类健康起着重要作用。然而，果汁中也可能含有痕量的潜在有毒元素，从而带来健康风险。
本工作旨在开发一种基于新型生物可降解杂化材料（BHM）预浓缩铅的分析方法，该材料由红球菌（Rhodococcus erythropolis）AW3 细菌和油菜毛状根组成。
将 BHM 应用于在线固相萃取（SPE）系统，通过电热原子吸收光谱法（ETAAS）测定果汁中的铅。
研究了关键参数对铅保留的影响。在最佳实验条件下，实现了高于 99.9%的萃取效率和 62.5 的富集因子。BHM 的动态容量为 36mg/g，有利于至少进行八次生物吸附-解吸循环后再使用该柱。对 5mL 样品进行预浓缩时，LOD 和 LOQ 分别为 5.0 和 16.5ng/L 铅。在 1μg/L 铅和 n=10 的情况下，RSD 为 4.8%。
所开发的方法适用于不同类型果汁中铅的测定。
一种基于生物杂化吸附剂使用的新型微萃取程序。痕量铅的高灵敏度测定。果汁样品中 Pb 的分析。用于 Pb 测定的环保型微萃取技术。
