Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia.
P. A. Hertsen Moscow Oncology Research Institute - Affiliated Branch of National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2023 May;175(1):63-66. doi: 10.1007/s10517-023-05812-3. Epub 2023 Jun 20.
The development of new drugs for the treatment of HIV infection requires testing of their efficacy in a relevant animal model, such as humanized mice, which, unfortunately, are not yet available in Russia. In the present study, we have developed conditions for the humanization of immunodeficient NSG mice with human hematopoietic stem cells. Humanized animals generated during the study showed a high degree of chimerism and harbored repopulation of the entire range of human lymphocytes required for HIV replication in the blood and organs. Inoculation of these mice with HIV-1 virus led to stable viremia, which was confirmed by the presence of viral RNA in blood plasma throughout the entire period of observation and proviral DNA in the organs of animals 4 weeks after HIV infection.
治疗 HIV 感染的新药的开发需要在相关的动物模型中测试其疗效，例如人源化小鼠，但遗憾的是，俄罗斯目前还没有这种模型。在本研究中，我们已经为人源化免疫缺陷 NSG 小鼠建立了用人造血干细胞进行人源化的条件。研究过程中产生的人源化动物表现出高度的嵌合性，并重新填充了血液和器官中 HIV 复制所需的整个范围的人类淋巴细胞。将这些小鼠接种 HIV-1 病毒后，导致稳定的病毒血症，这通过整个观察期间血浆中存在病毒 RNA 和 HIV 感染后 4 周动物器官中的前病毒 DNA 得到证实。
