Bernard D, Peakman M, Hayday A C
King's College London, Department of Immunobiology, London, UK.
Clin Exp Immunol. 2008 Jun;152(3):406-14. doi: 10.1111/j.1365-2249.2008.03659.x. Epub 2008 Apr 24.
Studies on physiology and pathology as they relate to the immune system draw heavily upon rodent models. With the increasing impetus provided by initiatives in translational medicine, the demand for ever more sophisticated, 'humanized' murine models is greater than ever. However, the design and implementation of studies in such mice is far from trivial. Here we provide a technical perspective on the increasing interest in developing humanized mice. We give examples of primary data starting with the routine procurement of human donor material, through CD34(+) cell purification prior to engraftment to injection into immunocompromised mice. Our goal is to provide practical advice to the many investigators who may be commencing or considering such studies.
与免疫系统相关的生理学和病理学研究在很大程度上依赖于啮齿动物模型。随着转化医学计划提供的动力不断增加，对更加复杂的“人源化”小鼠模型的需求比以往任何时候都更大。然而，在此类小鼠中开展研究的设计和实施绝非易事。在此，我们从技术角度阐述了对开发人源化小鼠日益增长的兴趣。我们给出了原始数据的示例，从常规获取人类供体材料开始，到移植前对CD34(+)细胞进行纯化，再到注射到免疫缺陷小鼠体内。我们的目标是为许多可能正在开展或考虑此类研究的研究人员提供实用建议。
