Unidade de Pesquisa Urogenital - Universidade do Estado do Rio de Janeiro - Uerj, Rio de Janeiro, RJ, Brasil.
Int Braz J Urol. 2023 Nov-Dec;49(6):749-756. doi: 10.1590/S1677-5538.IBJU.2023.9909.
To analyze the histology and distribution of abdominal testicular vessels in human fetuses Patients and Methods: We studied 19 fetuses (34 testes) ranging in age from 12 to 19 weeks post-conception. The fetuses were evaluated regarding crown-rump length (CRL), total length (TL) and body weight immediately before dissection. Each testis was dissected and embedded in paraffin, from which 5 μm thick sections were obtained and stained with Masson's trichrome and Anti-CD31 antibody to quantify the vessels. The stereological analysis was carried out with the Image Pro and Image J programs, using a grid to determine volumetric densities (Vv). Means were statistically compared using the unpaired T-test (p<0.05).
The fetuses presented mean weight of 222.5g, mean CRL of 15.3 cm and mean TL of 23.2 cm. All testes were in the abdominal position. The mean percentage of vessels (Vv) in the upper portion of the testis was 7.6% (4.6 to 15%) and in the lower portion the mean was 5.11% (2.3 to 9.8%), with a significant difference (p=0.0001). In the analysis between the upper portion of the right and left testes (p=0.99) and in the analysis of the lower portion of the right and left testes (p=0.83), we did not observe significant differences.
The upper portion of the abdominal testis in human fetuses had a higher concentration of vessels than the lower portion. These results suggest that manipulation of the lower end of the testis during Fowler-Stephens surgery should be avoided in order to preserve the collateral circulation.
分析人类胎儿腹部睾丸血管的组织学和分布。
我们研究了 19 例（34 个睾丸）从受孕后 12 周到 19 周的胎儿。在进行解剖之前，对每个胎儿的头臀长（CRL）、总长度（TL）和体重进行了评估。将每个睾丸解剖并嵌入石蜡中，从其中获取 5μm 厚的切片，并用 Masson 三色和抗 CD31 抗体染色以量化血管。使用图像 Pro 和图像 J 程序进行立体学分析，使用网格来确定体积密度（Vv）。使用未配对 T 检验（p<0.05）对平均值进行统计学比较。
胎儿的平均体重为 222.5g，平均 CRL 为 15.3cm，平均 TL 为 23.2cm。所有睾丸均位于腹部位置。睾丸上部的血管（Vv）平均百分比为 7.6%（4.6 至 15%），下部平均为 5.11%（2.3 至 9.8%），差异有统计学意义（p=0.0001）。在右侧和左侧睾丸上部之间的分析（p=0.99）和右侧和左侧睾丸下部之间的分析（p=0.83）中，我们没有观察到显著差异。
人类胎儿腹部睾丸的上部比下部有更高浓度的血管。这些结果表明，在 Fowler-Stephens 手术中应避免操作睾丸的下端，以保留侧支循环。
