Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, National Engineering Research Center for Sugarcane, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Agric Food Chem. 2023 Jul 5;71(26):10004-10017. doi: 10.1021/acs.jafc.3c01886. Epub 2023 Jun 20.
In plants, lysine acetylation (Kac), 2-hydroxyisobutyrylation (Khib), and lysine lactylation (Kla), the three new types of post-translational modification (PTM), play very important roles in growth, development, and resistance to adverse environmental stresses. Herein, we report the first global acetylome, 2-hydroxyisobutyrylome, and lactylome in sugarcane. A total of 8573 Kac, 4637 Khib, and 215 Kla sites across 3903, 1507, and 139 modified proteins were identified. Besides, homology analyses revealed the Kac, Khib, and Kla sites on histones were conserved between sugarcane and rice or poplar. Functional annotations demonstrated that the Kac, Khib, and Kla proteins were mainly involved in energy metabolism. In addition, a number of modified transcription factors and stress-related proteins, which were constitutively expressed in different tissues of sugarcane and induced by drought, cold or stress, were identified. Finally, a proposed working mode on how PTM functions in sugarcane was depicted. We thus concluded that PTM should play a role in sugarcane growth, development, and response to biotic and abiotic stresses, but the mechanisms require further investigation. The present study provided the all-new comprehensive profile of proteins Kac, Khib, and Kla and a new perspective to understand the molecular mechanisms of protein PTMs in sugarcane.
在植物中，赖氨酸乙酰化（Kac）、2-羟基异丁酰化（Khib）和赖氨酸乳酰化（Kla）这三种新型的翻译后修饰（PTM）在生长、发育和抵抗不利环境胁迫方面发挥着非常重要的作用。在此，我们报道了甘蔗中第一个乙酰组、2-羟基异丁酰组和乳酰组。共鉴定了 3903 个蛋白中的 8573 个 Kac、1507 个蛋白中的 4637 个 Khib 和 139 个蛋白中的 215 个 Kla 位点。此外，同源性分析表明，甘蔗和水稻或杨树之间组蛋白上的 Kac、Khib 和 Kla 位点是保守的。功能注释表明，Kac、Khib 和 Kla 蛋白主要参与能量代谢。此外，还鉴定了一些修饰的转录因子和与应激相关的蛋白，这些蛋白在甘蔗不同组织中持续表达，并能被干旱、寒冷或胁迫诱导。最后，提出了一个关于 PTM 如何在甘蔗中发挥作用的工作模式。因此，我们得出结论，PTM 应该在甘蔗的生长、发育和对生物和非生物胁迫的反应中发挥作用，但机制需要进一步研究。本研究提供了蛋白质 Kac、Khib 和 Kla 的全新综合图谱，为理解甘蔗中蛋白质 PTM 的分子机制提供了新的视角。
