Matson R S, Goheen S C
J Chromatogr. 1986 May 30;359:285-95. doi: 10.1016/0021-9673(86)80082-6.
We have studied the effects of various detergents and their concentration during human erythrocyte membrane solubilization. Detergents were selected on the basis of their low UV absorption at 280 nm, making them useful for high-performance liquid chromatography. High performance size-exclusion chromatography was then utilized to monitor the efficiency of solubilization. Sodium dodecyl sulfate solubilized more of the erythrocyte membrane proteins than any of the other detergents studied. 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfate solubilized some, but Tween 20 and reduced Triton X-100 solubilized fewer of the human erythrocyte membrane proteins. In conclusion, high-performance size exclusion chromatography provides a rapid method for determining whether membrane proteins have been effectively solubilized.
我们研究了各种去污剂及其浓度在人红细胞膜溶解过程中的作用。根据去污剂在280nm处的低紫外吸收特性进行选择，使其适用于高效液相色谱。然后利用高效尺寸排阻色谱法监测溶解效率。十二烷基硫酸钠溶解的红细胞膜蛋白比所研究的任何其他去污剂都多。3-[(3-胆酰胺丙基)-二甲基铵]-1-丙烷磺酸盐能溶解一些蛋白，但吐温20和还原型 Triton X-100溶解的人红细胞膜蛋白较少。总之，高效尺寸排阻色谱法提供了一种快速方法来确定膜蛋白是否已被有效溶解。
