Gooding D L, Schmuck M N, Nowlan M P, Gooding K M
J Chromatogr. 1986 May 30;359:331-7. doi: 10.1016/0021-9673(86)80087-5.
The chromatographic behavior of five proteins on hydrophobic interaction matrices having six different ligand arms was investigated using gradient elution with ammonium sulfate and ammonium acetate buffers at two pH values. The nature of the mobile phase and/or the ligand chain arm of the matrix was found to have substantial effect on the resolution, retention, and selectivity. Ovalbumin was moderately or highly retained with ammonium sulfate on all columns; however, with ammonium acetate, ovalbumin was not retained on SynChropak Hydroxypropyl and Propyl columns. Chromatographic conditions developed for analytical hydrophobic interaction chromatography columns containing 6.5-micron packings were adapted to preparative columns packed with 30-micron SynChroprep packings for the separation of serum components. Dynamic load capacities were 4-13 mg of ovalbumin per ml of column volume.
使用硫酸铵和醋酸铵缓冲液在两个pH值下进行梯度洗脱，研究了五种蛋白质在具有六种不同配体臂的疏水相互作用基质上的色谱行为。发现流动相的性质和/或基质的配体链臂对分离度、保留率和选择性有显著影响。在所有柱上，卵清蛋白用硫酸铵适度或高度保留；然而，使用醋酸铵时，卵清蛋白在SynChropak羟丙基柱和丙基柱上不被保留。为含有6.5微米填料的分析型疏水相互作用色谱柱开发的色谱条件适用于填充30微米SynChroprep填料的制备柱，用于血清成分的分离。动态负载量为每毫升柱体积4 - 13毫克卵清蛋白。
