Olajos S, Sztaniszláv D
J Chromatogr. 1986 May 28;378(1):155-62. doi: 10.1016/s0378-4347(00)80708-2.
A sensitive gas chromatographic method was developed for the determination of Trelibet, 1-benzyl-4-(2'-pyridinecarbonyl)piperazine, and of its major metabolites in biological fluids. The compounds were extracted as bases into dichloromethane, and the extracts were analysed by a dimethylsilicone capillary column with a nitrogen-phosphorus flame-ionization detector. The lower limit of detection was 1 ng/ml for Trelibet and 5 ng/ml for the metabolites. Peak-area ratios of the compounds and internal standard were linearly correlated to their plasma concentrations between 1 and 1000 ng/ml. The method was used for quantification of Trelibet and two of its metabolites in depressed patients after oral administration of a single dose of 200 mg of Trelibet. Concentration data measured in plasma and urine showed that the method is sensitive enough to monitor concentrations both for pharmacokinetic studies and for plasma steady-state levels daily.
建立了一种灵敏的气相色谱法，用于测定曲唑酮（1-苄基-4-(2'-吡啶甲酰基)哌嗪）及其在生物流体中的主要代谢物。这些化合物以碱的形式萃取到二氯甲烷中，萃取物通过带有氮磷火焰离子化检测器的二甲基硅氧烷毛细管柱进行分析。曲唑酮的检测下限为1 ng/ml，代谢物的检测下限为5 ng/ml。化合物与内标的峰面积比在1至1000 ng/ml之间与其血浆浓度呈线性相关。该方法用于口服单剂量200 mg曲唑酮后抑郁症患者体内曲唑酮及其两种代谢物的定量分析。血浆和尿液中测得的浓度数据表明，该方法灵敏度足以监测药代动力学研究以及每日血浆稳态水平的浓度。
