Pritz M B, Stritzel M E
J Comp Neurol. 1986 Jul 1;249(1):1-12. doi: 10.1002/cne.902490102.
Projections to the dorsal column nucleus were investigated in the reptile Caiman crocodilus following application of horseradish peroxidase (HRP) crystals to the cervical spinal cord after dorsal myelotomy. Brains were processed for HRP histochemistry by use of tetramethylbenzidine as the chromogen. Injection of the dorsal funiculus alone labelled axons that terminated solely in the ipsilateral dorsal column nucleus. Application of HRP that extended beyond the dorsal funiculus labelled axons that terminated in brainstem areas in addition to the dorsal column nucleus depending upon the injection site. Retrogradely labelled neurons in the brainstem were seen only after HRP injections that extended beyond the dorsal funiculus and varied depending upon the injection site. Dorsal funiculus fibers travel rostrally and turn laterally to reach more anterior portions of the dorsal column nucleus. Fibers peel off in an oblique ventrolateral fashion to terminate in the posterior part of the nucleus where boutons are intermixed with fibers. The anterior portion of the nucleus contains a greater proportion of axon terminals as compared to fibers than does the posterior part. A graphic reconstruction of the dorsal column nucleus was made by superimposing serial sections to view the nucleus in transverse, sagittal, and horizontal planes.
在对爬行动物凯门鳄进行背侧脊髓切开术后，将辣根过氧化物酶（HRP）晶体应用于颈脊髓后，研究了其向背柱核的投射。使用四甲基联苯胺作为显色剂对大脑进行HRP组织化学处理。单独注射背侧索标记的轴突仅终止于同侧背柱核。根据注射部位，超出背侧索应用HRP标记的轴突除了终止于背柱核外，还终止于脑干区域。仅在超出背侧索的HRP注射后才可见脑干中逆行标记的神经元，且其数量因注射部位而异。背侧索纤维向头侧走行并转向外侧，以到达背柱核更靠前的部分。纤维以斜向腹外侧的方式分支，终止于核的后部，在那里终扣与纤维混合。与后部相比，核的前部轴突终末的比例高于纤维。通过叠加连续切片制作了背柱核的图形重建，以在横切面、矢状面和水平面观察该核。
