College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, 450002, China.
Yellow River Institute of Hydraulic Research, Yellow River Conservancy Commission, Zhengzhou, 450003, China.
Plant Cell Rep. 2023 Sep;42(9):1433-1452. doi: 10.1007/s00299-023-03040-7. Epub 2023 Jun 21.
ER stress-responsive miRNAs, tae-miR164, tae-miR2916, and tae-miR396e-5p, are essential in response to abiotic stress. Investigating ER stress-responsive miRNAs is necessary to improve plant tolerance to environmental stress. MicroRNAs (miRNAs) play vital regulatory roles in plant responses to environmental stress. Recently, the endoplasmic reticulum (ER) stress pathway, an essential signalling pathway in plants in response to adverse conditions, has been widely studied in model plants. However, miRNAs associated with ER stress response remain largely unknown. Using high-throughput sequencing, three ER stress-responsive miRNAs, tae-miR164, tae-miR2916, and tae-miR396e-5p were identified, and their target genes were confirmed. These three miRNAs and their target genes actively responded to dithiothreitol, polyethylene glycol, salt, heat, and cold stresses. Furthermore, in some instances, the expression patterns of the miRNAs and their corresponding target genes were contrasting. Knockdown of tae-miR164, tae-miR2916, or tae-miR396e-5p using a barley stripe mosaic virus-based miRNA silencing system substantially enhanced the tolerance of wheat plants to drought, salt, and heat stress. Under conditions involving these stresses, inhibiting the miR164 function by using the short tandem target mimic approach in Arabidopsis thaliana resulted in phenotypes consistent with those of miR164-silenced wheat plants. Correspondingly, overexpression of tae-miR164 in Arabidopsis resulted in a decreased tolerance to drought stress and, to some extent, a decrease in tolerance to salt and high temperature. These results revealed that tae-miR164 plays a negative regulatory role in wheat/Arabidopsis in response to drought, salt, and heat stress. Taken together, our study provides new insights into the regulatory role of ER stress-responsive miRNAs in abiotic stress responses.
内质网应激响应 miRNA，tae-miR164、tae-miR2916 和 tae-miR396e-5p，对于应对非生物胁迫至关重要。研究内质网应激响应 miRNA 对于提高植物对环境胁迫的耐受性是必要的。miRNAs（miRNAs）在植物应对环境胁迫的过程中发挥着重要的调节作用。最近，内质网（ER）应激途径作为植物应对不利条件的一个重要信号途径，在模式植物中得到了广泛的研究。然而，与 ER 应激响应相关的 miRNAs 仍然知之甚少。利用高通量测序，鉴定出三个 ER 应激响应 miRNA，tae-miR164、tae-miR2916 和 tae-miR396e-5p，并验证了它们的靶基因。这三个 miRNA 和它们的靶基因积极响应二硫苏糖醇、聚乙二醇、盐、热和冷胁迫。此外，在某些情况下，miRNA 及其相应靶基因的表达模式是相反的。利用大麦条纹花叶病毒（BSMV）介导的 miRNA 沉默系统沉默 tae-miR164、tae-miR2916 或 tae-miR396e-5p，显著增强了小麦植株对干旱、盐和热胁迫的耐受性。在这些胁迫条件下，在拟南芥中使用短串联靶标模拟方法抑制 miR164 功能导致的表型与 miR164 沉默的小麦植株一致。相应地，在拟南芥中过表达 tae-miR164 导致其对干旱胁迫的耐受性降低，在一定程度上降低了对盐和高温的耐受性。这些结果表明，tae-miR164 在小麦/拟南芥应对干旱、盐和热胁迫中发挥负调节作用。总之，本研究为内质网应激响应 miRNA 在非生物胁迫响应中的调节作用提供了新的见解。
