Uhnoo I, Wadell G, Svensson L, Olding-Stenkvist E, Ekwall E, Mölby R
J Infect. 1986 Jul;13(1):73-89. doi: 10.1016/s0163-4453(86)92348-0.
In a prospective 1-year study, 144 children attending or admitted to hospital and 272 children outside hospital with acute gastro-enteritis and 200 controls were investigated by a broad panel of diagnostic methods for enteropathogenic agents in the faeces and for related antibody responses. Enteropathogens were identified in 77% of the inpatients, 63% of the outpatients and 8% of the controls. Rotavirus and Yersinia enterocolitica were detected significantly more often among inpatients. Altogether, viral, bacterial and parasitic agents were found in 58%, 14% and 1% of diarrhoeal patients, respectively. The isolation of more than one pathogenic agent was uncommon (6.5%). Rotavirus (45%) and enteric adenoviruses 40 and 41 (7.9%) predominated among the viruses, while Campylobacter jejuni (4.8%) was most common among the bacteria. Clostridium difficile and/or its cytotoxin, which were found in 14% of the children with gastroenteritis and in 15% of the controls, were significantly associated with antibiotic therapy but not with gastro-intestinal illness. Diarrhoeal infections of unknown aetiology exhibited a seasonal peak in the autumn. The duration of excretion of enteropathogens was investigated. Rotavirus particles were detectable by solid-phase immune electron microscopy for 14-25 days after the diarrhoea had ceased. Transmission of rotavirus and bacterial pathogens within families was studied also.
在一项为期1年的前瞻性研究中，采用一系列广泛的诊断方法，对144名住院或入院的儿童、272名患有急性肠胃炎的非住院儿童以及200名对照儿童的粪便中肠道病原体及相关抗体反应进行了调查。住院患者中77%、门诊患者中63%以及对照儿童中8%检测到肠道病原体。住院患者中轮状病毒和小肠结肠炎耶尔森菌的检出率明显更高。腹泻患者中，病毒、细菌和寄生虫病原体的检出率分别为58%、14%和1%。分离出一种以上病原体的情况并不常见（6.5%）。病毒中轮状病毒（45%）以及肠道腺病毒40型和41型（7.9%）占主导，而细菌中最常见的是空肠弯曲菌（4.8%）。艰难梭菌和/或其细胞毒素在14%的肠胃炎儿童和15%的对照儿童中被发现，它们与抗生素治疗显著相关，但与胃肠道疾病无关。病因不明的腹泻感染在秋季出现季节性高峰。对肠道病原体的排泄持续时间进行了调查。腹泻停止后，通过固相免疫电子显微镜可在14 - 25天内检测到轮状病毒颗粒。还研究了轮状病毒和细菌病原体在家庭内的传播情况。
