Kubota K, Kasai T, Iwasaki H, Sekiya S, Takamizawa H, Tenjin Y
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Jun;38(6):863-70.
Hematoporphyrin derivative (HpD) is known to have an affinity for tumor cells. Photoradiation therapy (PRT), in which HpD is activated by an argon dye laser, is not always effective in treating all gynecological malignancies. Fundamental studies were performed to elucidate the mechanism acting on two cell lines and to evaluate efficiency when applied to them. Two cell lines established from endometrial adenocarcinoma and uterine choriocarcinoma were used in this study. There were apparent differences in 3H-thymidine incorporation on two cell lines treated by single HpD. In both cell lines, single HpD has some effects on neutral red uptake depending on the HpD concentration and length of treatment time. In PRT, a proportional increase in effects is obtained by extending the irradiation time. More than 90% effectiveness is obtained by pretreatment with HpD 40 micrograms/ml and irradiation for 3 minutes or more. On the DNA patterns of the cells, a shift in the histogram peak to diploid and a remarkable decrease in the number of high ploidy cells above tetraploidy occurred but were not so noteworthy in these two cell lines as in SKG-1 cells.
血卟啉衍生物（HpD）已知对肿瘤细胞具有亲和力。光辐射疗法（PRT）中，HpD由氩染料激光激活，但该疗法在治疗所有妇科恶性肿瘤时并不总是有效。我们进行了基础研究，以阐明对两种细胞系起作用的机制，并评估将其应用于这些细胞系时的效率。本研究使用了从子宫内膜腺癌和子宫绒毛膜癌建立的两种细胞系。在单一HpD处理的两种细胞系中，3H-胸腺嘧啶核苷掺入存在明显差异。在两种细胞系中，单一HpD对中性红摄取都有一定影响，这取决于HpD浓度和处理时间长度。在PRT中，通过延长照射时间可按比例增加效果。用40微克/毫升的HpD预处理并照射3分钟或更长时间可获得超过90%的有效性。在细胞的DNA图谱上，直方图峰值向二倍体偏移，四倍体以上的高倍体细胞数量显著减少，但在这两种细胞系中不如在SKG-1细胞中那么明显。
