鼻腔内翻性乳头状瘤的上皮紧密连接异常。

Epithelial Tight Junction Anomalies in Nasal Inverted Papilloma.

机构信息

Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, China.

Department of Otolaryngology, Infectious Diseases Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore.

出版信息

Laryngoscope. 2024 Feb;134(2):552-561. doi: 10.1002/lary.30845. Epub 2023 Jun 22.

DOI:10.1002/lary.30845

PMID:37345652

Abstract

OBJECTIVES

As a critical component of the epithelial barrier, tight junctions (TJs) are essential in nasal mucosa against pathogen invasion. However, the function of TJs has rarely been reported in nasal inverted papilloma (NIP). This study aims to investigate the potential factors of TJs' abnormality in NIP.

METHODS

We assessed the expression of ZO-1, occludin, claudin-1, claudin-3, and claudin-7 in healthy controls and NIP by real-time quantitative polymerase chain reaction and immunofluorescent staining. The correlation between TJs expression and neutrophil count, T 1/T 2/T 17 and regulatory T cell biomarkers, and the proportion of nasal epithelial cells was investigated.

RESULTS

Upregulation of ZO-1, occludin, claudin-1, and claudin-7, along with downregulation of claudin-3, was found in NIP compared to control (all p < 0.05). An abnormal proportion with a lower number of ciliated cells (control vs. NIP: 37.60 vs. 8.67) and goblet cells (12.52 vs. 0.33) together with a higher number of basal cells (45.58 vs. 124.00) in NIP. Meanwhile, claudin-3 was positively correlated with ciliated and goblet cells (all p < 0.01). Additionally, neutrophils were excessively infiltrated in NIP, negatively correlated with ZO-1, but positively with claudin-3 (all p < 0.05). Furthermore, FOXP3, IL-10, TGF-β1, IL-5, IL-13, and IL-22 levels were induced in NIP (all p < 0.01). Occludin level was negatively correlated with IL-10, IL-5, IL-13, and IL-22, whereas ZO-1 was positively with TGF-β1 (all p < 0.05).

CONCLUSION

Nasal epithelial barrier dysfunction with TJs anomalies is commonly associated with abnormal proliferation and differentiation of epithelial cells and imbalance of immune and inflammatory patterns in NIP.

LEVEL OF EVIDENCE

NA Laryngoscope, 134:552-561, 2024.

摘要

目的

紧密连接（TJs）作为上皮屏障的关键组成部分，对于鼻黏膜抵抗病原体入侵至关重要。然而，TJs 的功能在鼻腔内翻性乳头状瘤（NIP）中鲜有报道。本研究旨在探讨 NIP 中 TJs 异常的潜在因素。

方法

通过实时定量聚合酶链反应和免疫荧光染色评估健康对照组和 NIP 中 ZO-1、occludin、claudin-1、claudin-3 和 claudin-7 的表达。研究 TJs 表达与中性粒细胞计数、T1/T2/T17 和调节性 T 细胞生物标志物以及鼻上皮细胞比例之间的相关性。

结果

与对照组相比，NIP 中 ZO-1、occludin、claudin-1 和 claudin-7 的表达上调，而 claudin-3 的表达下调（均 p<0.05）。NIP 中存在异常比例的纤毛细胞（对照组 vs. NIP：37.60% vs. 8.67%）和杯状细胞（12.52% vs. 0.33%），同时基底细胞数量增加（45.58% vs. 124.00%）。此外，claudin-3 与纤毛细胞和杯状细胞呈正相关（均 p<0.01）。同时，NIP 中过度浸润中性粒细胞，与 ZO-1 呈负相关，但与 claudin-3 呈正相关（均 p<0.05）。此外，NIP 中 FOXP3、IL-10、TGF-β1、IL-5、IL-13 和 IL-22 水平升高（均 p<0.01）。occludin 水平与 IL-10、IL-5、IL-13 和 IL-22 呈负相关，而 ZO-1 与 TGF-β1 呈正相关（均 p<0.05）。