Lal Priyanka, Tiwari Rahul Kumar, Kumar Awadhesh, Altaf Muhammad Ahsan, Alsahli Abdulaziz Abdullah, Lal Milan Kumar, Kumar Ravinder
Department of Agricultural Economics and Extension, School of Agriculture, Lovely Professional University, Phagwara, India.
ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh, India.
Front Plant Sci. 2023 Jun 6;14:1129714. doi: 10.3389/fpls.2023.1129714. eCollection 2023.
The discovery of RT-PCR-based pathogen detection and gene expression analysis has had a transformative impact on the field of plant protection. This study aims to analyze the global research conducted between 2001 and 2021, focusing on the utilization of RT-PCR techniques for diagnostic assays and gene expression level studies. By retrieving data from the 'Dimensions' database and employing bibliometric visualization software, this analysis provides insights into the major publishing journals, institutions involved, leading journals, influential authors, most cited articles, and common keywords.
The 'Dimensions' database was utilized to retrieve relevant literature on RT-PCR-based pathogen detection. Fourteen distinct search queries were employed, and the resulting dataset was analyzed for trends in scholarly publications over time. The bibliometric visualization software facilitated the identification of major publishing journals, institutions, leading journals, influential authors, most cited articles, and common keywords. The study's search query was based on the conjunction 'AND', ensuring a comprehensive analysis of the literature.
The analysis revealed a significant increase in the number of scholarly publications on RT-PCR-based pathogen detection over the years, indicating a growing interest and investment in research within the field. This finding emphasizes the importance of ongoing investigation and development, highlighting the potential for further advancements in knowledge and understanding. In terms of publishing journals, Plos One emerged as the leading journal, closely followed by BMC Genomics and Phytopathology. Among the highly cited journals were the European Journal of Plant Pathology, BMC Genomics, and Fungal Genetics and Biology. The publications with the highest number of citations and publications were associated with the United Nations and China. Furthermore, a network visualization map of co-authorship analysis provided intriguing insights into the collaborative nature of the research. Out of 2,636 authors analyzed, 50 surpassed the level threshold, suggesting active collaboration among researchers in the field.
Overall, this bibliometric analysis demonstrates that the research on RT-PCR-based pathogen detection is thriving. However, there is a need for further strengthening using modern diagnostic tools and promoting collaboration among well-equipped laboratories. The findings underscore the significance of RT-PCR-based pathogen detection in plant protection and highlight the potential for continued advancements in this field. Continued research and collaboration are vital for enhancing knowledge, developing innovative diagnostic tools, and effectively protecting plants from pathogens.
基于逆转录聚合酶链反应（RT-PCR）的病原体检测和基因表达分析的发现，对植物保护领域产生了变革性影响。本研究旨在分析2001年至2021年间开展的全球研究，重点关注RT-PCR技术在诊断检测和基因表达水平研究中的应用。通过从“Dimensions”数据库检索数据并使用文献计量可视化软件，该分析提供了关于主要出版期刊、参与机构、领先期刊、有影响力的作者、被引次数最多的文章以及常见关键词的见解。
利用“Dimensions”数据库检索基于RT-PCR的病原体检测的相关文献。采用了14个不同的搜索查询，并对所得数据集进行分析，以了解学术出版物随时间的趋势。文献计量可视化软件有助于识别主要出版期刊、机构、领先期刊、有影响力的作者、被引次数最多的文章以及常见关键词。该研究的搜索查询基于“AND”连词，确保对文献进行全面分析。
分析显示，多年来基于RT-PCR的病原体检测的学术出版物数量显著增加，表明该领域对研究的兴趣和投入不断增长。这一发现强调了持续调查和开发的重要性，突出了在知识和理解方面进一步进步的潜力。在出版期刊方面，《公共科学图书馆·综合》（Plos One）成为领先期刊，紧随其后的是《BMC基因组学》和《植物病理学》。被引次数较高的期刊包括《欧洲植物病理学杂志》《BMC基因组学》以及《真菌遗传学与生物学》。被引次数和出版物数量最多的出版物与联合国和中国有关。此外，合著分析的网络可视化地图为该研究的合作性质提供了有趣的见解。在分析的2636位作者中，有50位超过了水平阈值，表明该领域的研究人员之间积极合作。
总体而言，这项文献计量分析表明，基于RT-PCR的病原体检测研究正在蓬勃发展。然而，需要进一步加强使用现代诊断工具，并促进设备完善的实验室之间开展合作。研究结果强调了基于RT-PCR的病原体检测在植物保护中的重要性，并突出了该领域持续进步的潜力。持续的研究与合作对于增进知识、开发创新诊断工具以及有效保护植物免受病原体侵害至关重要。
