Hoogenkamp Michel A, Mazurel Danuta, Deutekom-Mulder Elly, de Soet Johannes J
Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Biofilm. 2023 May 25;5:100132. doi: 10.1016/j.bioflm.2023.100132. eCollection 2023 Dec.
The water systems inside a dental unit are known to be contaminated with a multi-kingdom biofilm encompassing bacteria, fungi, viruses and protozoa. Aerosolization of these micro-organisms can potentially create a health hazard for both dental staff and the patient. Very little is known on the efficacy of dental unit disinfection products against amoeba. In this study we have examined the effect of four different treatment regimens, with the hydrogen peroxide (HO) containing product Oxygenal, on an multi-kingdom dental unit water system (DUWS) biofilm. The treatment efficacy was assessed in time using heterotrophic plate counts, the bacterial 16S rDNA, fungal 18S rDNA gene load and the number of genomic units for spp. the amoeba The results indicated that a daily treatment of the DUWS with a low dose HO (0.02% for 5 h), combined with a weekly shock dose (0.25% HO, 30 min) is necessary to reduce the heterotrophic plate count of a severely contaminated DUWS (>10 CFU.mL) to below 100 CFU.mL. A daily treatment with a low dose hydrogen peroxide alone, is sufficient for the statistically significant reduction of the total amount of bacterial 16S rDNA gene, spp. and load (p < 0.005). Also shown is that even though hydrogen peroxide does not kill the trophozoite nor the cysts of , it does however result in the detachment of the trophozoite form of this amoeba from the DUWS biofilm and hereby ultimately removing the amoeba from the system.
牙科设备内部的水系统已知被包含细菌、真菌、病毒和原生动物的多界生物膜污染。这些微生物的雾化可能会对牙科工作人员和患者都造成健康危害。关于牙科设备消毒产品对变形虫的功效了解甚少。在本研究中，我们研究了四种不同的处理方案，使用含过氧化氢（HO）的产品Oxygenal，对多界牙科设备水系统（DUWS）生物膜的影响。使用异养平板计数、细菌16S rDNA、真菌18S rDNA基因载量以及变形虫属的基因组单位数量及时评估处理效果。结果表明，对于严重污染的DUWS（>10⁵ CFU.mL⁻¹），每天用低剂量HO（0.02%，5小时）处理，并结合每周一次的冲击剂量（0.25% HO，30分钟），才能将其异养平板计数降低到100 CFU.mL⁻¹以下。仅每天用低剂量过氧化氢处理，就足以使细菌16S rDNA基因总量、变形虫属和负载量在统计学上显著降低（p < 0.005）。还表明，尽管过氧化氢不会杀死变形虫的滋养体和包囊，但它确实会导致这种变形虫的滋养体形式从DUWS生物膜上脱离，从而最终从系统中去除变形虫。
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