van Noort M, Rommerts F F, van Amerongen A, Wirtz K W
J Endocrinol. 1986 Jun;109(3):R13-6. doi: 10.1677/joe.0.109r013.
In testis tissue from mature rats the non-specific lipid transfer protein (nsLTP), also called sterol carrier protein2 (SCP2), is concentrated in the Leydig cells and cannot be detected in Sertoli cells or germinal cells. Conclusions were reached after cell fractionation studies with normal testis tissue and after selective destruction of Leydig cells or germinal cells in vivo. The amount of nsLTP (SCP2) in testis tissue increased twofold 48 h after two daily injections of human chorionic gonadotrophin (100 i.u., s.c.) and decreased twofold after plasma luteinizing hormone levels were suppressed to almost undetectable levels with silicone elastomer implants containing testosterone. The specific localization in the Leydig cells and the luteinizing hormone-dependent cellular concentration of nsLTP/SCP2 support the possibility that this protein could play a role in the regulation of steroidogenesis by regulating the availability of cholesterol for the P450 side-chain cleavage enzyme in the mitochondria of Leydig cells.
在成熟大鼠的睾丸组织中，非特异性脂质转运蛋白（nsLTP），也称为固醇载体蛋白2（SCP2），集中在睾丸间质细胞中，在支持细胞或生殖细胞中无法检测到。这一结论是通过对正常睾丸组织进行细胞分级分离研究，以及在体内选择性破坏睾丸间质细胞或生殖细胞后得出的。每天两次注射人绒毛膜促性腺激素（100国际单位，皮下注射）48小时后，睾丸组织中nsLTP（SCP2）的量增加了两倍；而在用含睾酮的硅橡胶植入物将血浆促黄体生成素水平抑制到几乎无法检测的水平后，其含量降低了两倍。nsLTP/SCP2在睾丸间质细胞中的特异性定位以及其依赖促黄体生成素的细胞浓度支持了这样一种可能性，即该蛋白可能通过调节睾丸间质细胞线粒体中细胞色素P450侧链裂解酶的胆固醇供应，在类固醇生成的调节中发挥作用。
