McCaig C D
J Embryol Exp Morphol. 1986 Apr;93:121-31.
The orientation of developing myoblasts extending a bipolar axis in the presence of explanted myoblasts or whole notochord has been studied in vitro. Myoblasts tended to elongate perpendicular to the lines of diffusion of substances from these tissues. A slow-release source of agar impregnated with medium conditioned by segmented somitic myoblast or notochord also caused myoblasts to elongate perpendicular to the lines of diffusion from the source. Medium conditioned by neural tube cells or unsegmented mesoderm cells did not influence the orientation of myoblasts. It is concluded that somites and notochord release diffusible substances in vitro which are capable of directing the orientation of developing myoblasts. In vivo, a somite-derived material could play a role in determining the direction of myoblast elongation in the presomitic mesoderm. An interaction between somite and notochord-derived secretions could influence the rotation of presomitic myoblasts to form a segmented somite.
在体外研究了发育中的成肌细胞在植入的成肌细胞或完整脊索存在的情况下延伸双极轴的方向。成肌细胞倾向于垂直于来自这些组织的物质扩散线伸长。用节段性体节成肌细胞或脊索条件培养基浸渍的琼脂缓释源也会使成肌细胞垂直于来自该源的扩散线伸长。神经管细胞或未节段化中胚层细胞的条件培养基不影响成肌细胞的方向。得出的结论是，体节和脊索在体外释放可扩散物质，这些物质能够指导发育中的成肌细胞的方向。在体内，一种体节衍生物质可能在确定前体中胚层中成肌细胞伸长方向方面发挥作用。体节和脊索衍生分泌物之间的相互作用可能影响前体成肌细胞的旋转以形成节段性体节。
