Brunnekreeft J W, Leijnse B
J Clin Chem Clin Biochem. 1986 Jul;24(7):445-9. doi: 10.1515/cclm.1986.24.7.445.
We report an accurate method, based on capillary on-column gas chromatography, for determining triglycerides in human serum. After serum extraction and chemical hydrolysis, glycerol is directly measured by gas chromatography (GC). With our extraction method no free glycerol is extracted from serum. The accuracy of this method was compared with that of a method based on the original procedure of Carlson (J. Atheroscler. Res. 3, 334-336 (1963)) and which is standardized by the Centers for Disease Control (CDC, Atlanta, U.S.A.). Orthogonal regression analysis of the GC method (y) and the CDC reference method (X) resulted in y = 0.996 X + 0.000 with a correlation coefficient of 0.999. The variances of analytical data, collected over a two month period showed that, for the GC method, the within-day coefficient of variation (CV) was less than 1.43%; the between-day CV less than 1.35%. The data for the CDC method were less than 3.36% (within-day) and less than 6.38% (between-days). The CDC method is linear up to 3.7 mmol/l and the GC method to 22 mmol/l.
我们报告了一种基于毛细管柱上气相色谱法的准确方法，用于测定人血清中的甘油三酯。血清提取和化学水解后，通过气相色谱法（GC）直接测量甘油。采用我们的提取方法，血清中不会提取出游离甘油。将该方法的准确性与基于卡尔森原始方法（《动脉粥样硬化研究杂志》3, 334 - 336 (1963)）并由美国疾病控制中心（美国亚特兰大疾病预防控制中心）标准化的方法进行了比较。对气相色谱法（y）和疾病预防控制中心参考方法（X）进行正交回归分析，结果为y = 0.996X + 0.000，相关系数为0.999。在两个月期间收集的分析数据方差表明，对于气相色谱法，日内变异系数（CV）小于1.43%；日间CV小于1.35%。疾病预防控制中心方法的数据日内小于3.36%，日间小于6.38%。疾病预防控制中心方法在高达3.7 mmol/l时呈线性，气相色谱法在高达22 mmol/l时呈线性。
