Inoue T, Suzuki S
J Forensic Sci. 1986 Jul;31(3):1102-7.
Five extraction methods were examined for analysis of methamphetamine and its major metabolites in tissue samples. The extraction methods studied were an acetone extraction method, an ethanol extraction method, an ammonium sulfate method, dialysis, and a direct solvent extraction. Acetone, ethanol, and dialysis methods showed no interference from endogenous components using thin-layer chromatography and gas chromatography, and gave satisfactory recovery of methamphetamine, amphetamine, and p-hydroxymethamphetamine when added to rabbit liver. These methods, however, proved time-consuming. The ammonium sulfate method and direct solvent extraction method were simple and more rapid, but recovery of the polar metabolite was poor.
研究了五种提取方法用于分析组织样本中的甲基苯丙胺及其主要代谢物。所研究的提取方法包括丙酮提取法、乙醇提取法、硫酸铵法、透析法和直接溶剂萃取法。使用薄层色谱法和气相色谱法时，丙酮、乙醇和透析法未显示出内源性成分的干扰，并且向兔肝中添加甲基苯丙胺、苯丙胺和对羟基甲基苯丙胺时，回收率令人满意。然而，这些方法被证明耗时较长。硫酸铵法和直接溶剂萃取法简单且更快速，但极性代谢物的回收率较差。
