Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, Brazil.
Universidade Estadual de Campinas, Centro de Atenção Integral a Saúde da Mulher, Campinas, Brazil.
Innate Immun. 2023 Jul;29(5):71-82. doi: 10.1177/17534259231178592. Epub 2023 Jun 22.
Killer cell immunoglobulin-like receptors (KIR) exhibit extensive diversity, giving rise to different KIR profiles in populations worldwide. This study aimed to investigate the distribution of KIR genes and HLA ligands in a population from Campinas, southeastern Brazil (n = 292), and to compare their results with other populations. A comprehensive analysis of population-specific genes, genotype, and haplotype frequencies of KIR may facilitate a better understanding of their evolution and role in immunity. The genotyping of 16 KIR genes and HLA class I alleles was performed by the reverse sequence-specific oligonucleotide methodology using the Luminex platform (One Lambda, Inc., Canoga Park, CA). The framework genes were present in all individuals, with the most common non-framework KIR genes detected being KIR2DP1(96.6%), KIR2DL1(95.5%), KIR3DL1(94.5%), KIR2DS4(93.8%) and KIR2DL3(87.3%). KIR2DS1, KIR2DS3, KIR2DS5, and KIR3DS1 presented frequencies below 40%. KIR2DL2, KIR2DL5, and KIR2DS2 showed intermediate frequencies (between53% and 58%). The activating gene KIR2DS5 was the least common in this population (30.8%). Forty-five KIR profiles were found with the commonest being the homozygous A haplotype (27.4%). The distribution of KIR genes in the Brazilian population is similar to Caucasian European and Euro-descendant populations.
杀伤细胞免疫球蛋白样受体（KIR）表现出广泛的多样性，导致全球不同人群的 KIR 谱不同。本研究旨在调查巴西东南部坎皮纳斯（n = 292）人群的 KIR 基因和 HLA 配体分布，并将其结果与其他人群进行比较。对 KIR 基因、基因型和单倍型频率的人群特异性分析有助于更好地理解其在免疫中的进化和作用。使用 Luminex 平台（One Lambda，Inc.，Canoga Park，CA）通过反向序列特异性寡核苷酸方法对 16 个 KIR 基因和 HLA Ⅰ类等位基因进行基因分型。所有个体均存在框架基因，最常见的非框架 KIR 基因是 KIR2DP1（96.6%）、KIR2DL1（95.5%）、KIR3DL1（94.5%）、KIR2DS4（93.8%）和 KIR2DL3（87.3%）。KIR2DS1、KIR2DS3、KIR2DS5 和 KIR3DS1 的频率低于 40%。KIR2DL2、KIR2DL5 和 KIR2DS2 的频率（53%至 58%之间）处于中间水平。激活基因 KIR2DS5 在该人群中最不常见（30.8%）。共发现 45 种 KIR 表型，最常见的是纯合 A 单倍型（27.4%）。巴西人群的 KIR 基因分布与白种欧洲人和欧洲裔人群相似。
