Rudnick Cristiane Conceição Chagas, Franceschi Danilo Santana Alessio, Marangon Amanda Vansan, Guelsin Gláucia Andréia Soares, Sell Ana Maria, Visentainer Jeane Eliete Laguila
Laboratório de Imunogenética, Departamento de Análises Clínicas, Universidade Estadual de Maringá, PR, Brazil.
Hum Immunol. 2008 Dec;69(12):872-6. doi: 10.1016/j.humimm.2008.09.002. Epub 2008 Oct 9.
Killer cell immunoglobulin-like receptors (KIR) are encoded by polymorphic genes and have as binding human leukocyte antigen (HLA) class I molecules. The aim of this study was to investigate the distribution of KIR genes and inhibitory KIR/HLA pairs in a population from Southern Brazil, in the state of Paraná, and to compare the results with results from other populations. The genotyping of 16 KIR genes and HLA class I alleles of 289 unrelated individuals was accomplished by reverse sequence-specific oligonucleotide Luminex (One Lambda, Inc., Canoga Park, CA). This Brazilian population demonstrated several similarities to Caucasian populations with regard to the frequency of KIR genes. Thirty-eight genotypes were defined in which the most frequent was the homozygous haplotype A (33.2%). Therefore, it was possible to define two new genotypes. Most of the individuals demonstrated at least one inhibitory KIR/HLA pair. Two pairs were the most frequent (40.4%), followed by three pairs (38.2%), one pair (14.6%), and four pairs (6.4%). The KIR2DL2/3 + HLA-C1 pair was the most frequent (79.9%) and the least frequent pair was KIR3DL2 + HLA-A3/11 (25.0%). This study demonstrated the diversity of KIR genes in a population of Paraná, as well as the characteristic pattern of Caucasians with racial admixture, which enabled the definition of two new genotypes and the identification of one individual without the inhibitory KIR/HLA pair.
杀伤细胞免疫球蛋白样受体（KIR）由多态性基因编码，可与人白细胞抗原（HLA）I类分子结合。本研究的目的是调查巴西南部巴拉那州人群中KIR基因和抑制性KIR/HLA配对的分布，并将结果与其他人群的结果进行比较。通过反向序列特异性寡核苷酸Luminex（One Lambda公司，加利福尼亚州卡诺加公园）对289名无关个体的16个KIR基因和HLA I类等位基因进行基因分型。在KIR基因频率方面，该巴西人群与高加索人群表现出若干相似之处。定义了38种基因型，其中最常见的是纯合单倍型A（33.2%）。因此，有可能定义两种新的基因型。大多数个体表现出至少一对抑制性KIR/HLA配对。两对最为常见（40.4%），其次是三对（38.2%）、一对（14.6%）和四对（6.4%）。KIR2DL2/3 + HLA-C1配对最为常见（79.9%），最不常见的配对是KIR3DL2 + HLA-A3/11（25.0%）。本研究证明了巴拉那州人群中KIR基因的多样性，以及具有种族混合特征的高加索人的特征模式，这使得能够定义两种新的基因型，并识别出一名没有抑制性KIR/HLA配对的个体。
