Guinet R, Absi N
Ann Biol Clin (Paris). 1978;36(6):519-21.
The indirect fluorescent antibody (IFA) technique for specific IgM anti-Candida albicans was applied to 158 sera and compared with sucrose density gradient centrifugation. For detection of specific IgM results of both methods were in agreement for 157 sera (99.36%). Specific IgM titers determined with IFA on unfractionated sera followed those found on fractionated sera. The results show that specific IgM anti-C. albicans can be identified and titrated by the immunofluorescent technique.
将用于检测特异性抗白色念珠菌 IgM 的间接荧光抗体(IFA)技术应用于 158 份血清,并与蔗糖密度梯度离心法进行比较。对于特异性 IgM 的检测,两种方法对 157 份血清(99.36%)的检测结果一致。用 IFA 测定的未分级血清中的特异性 IgM 滴度与分级血清中的结果一致。结果表明,特异性抗白色念珠菌 IgM 可用免疫荧光技术进行鉴定和滴定。