果糖补充剂可改变实验性偏头痛大鼠的大脑代谢。

Fructose supplementation shifts rat brain metabolism in experimental migraine.

机构信息

PUCRS, Programa de Pós-Graduação em Biologia Celular e Molecular, Escola de Ciências da Saúde e da Vida, Porto Alegre, RS, Brazil; PUCRS, Curso de Graduação em Odontologia, Escola de Ciências da Saúde e da Vida, Porto Alegre, RS, Brazil.

PUCRS, Curso de Graduação em Odontologia, Escola de Ciências da Saúde e da Vida, Porto Alegre, RS, Brazil; PUCRS, Centro de Pesquisa em Toxicologia e Farmacologia, Escola de Ciências da Saúde e da Vida, Porto Alegre, RS, Brazil.

出版信息

Brain Res Bull. 2023 Aug;200:110694. doi: 10.1016/j.brainresbull.2023.110694. Epub 2023 Jun 21.

DOI:10.1016/j.brainresbull.2023.110694

PMID:37353036

Abstract

AIMS

We have previously demonstrated that fructose supplementation (FS), given in a scheme used for inducing metabolic syndrome (MS), elicited pain relief in the nitroglycerin (NTG)-elicited rat migraine model. Herein, we evaluated whether FS could reestablish the impaired metabolic pathways in NTG-injected rats.

MAIN METHODS

Male Wistar rats (N = 40) were divided into two groups for receiving 10 % FS or tap water. After 45 days, they were subdivided into NTG-injected (10 mg/kg; 15 days) or controls. After the fourth NTG injection, F-fluorodeoxyglucose ([F] FDG) micro-PET scanning was accomplished. The day after, euthanasia was performed, and blood was collected for glycemia and LDH analysis. The levels of energy molecules, TBARS, PGC-1α, and MCTS1 were evaluated in the brain cortices. The activated satellite glial cells (SGC) were assessed in the trigeminal ganglion (TG).

KEY FINDINGS

There were no variations of glycemia or LDH serum levels. NTG-injected rats showed a significant increase in glucose uptake in the hypothalamus (HT) vs. NTG-free rats. The FS-NTG group showed increased metabolism in the superior colliculus (SC) vs. the NTG group. Moreover, the glucose uptake was amplified in the inferior colliculus (IC) of the FS-NTG vs. FS group. The cortical inosine levels were significantly higher in FS-NTG rats vs. NTG or FS groups, with no changes in TBARS or MCTS1 levels, despite a minor decrease of PGC1-α contents in the FS+NTG group. Finally, there was a significant increase of activated SGC around TG in the FS-NTG rats.

SIGNIFICANCE

We provide novel evidence linking nutrition and metabolism with migraine.

摘要

目的

我们之前已经证明，在诱导代谢综合征（MS）的方案中补充果糖（FS）可在硝化甘油（NTG）诱导的大鼠偏头痛模型中缓解疼痛。在此，我们评估 FS 是否可以恢复 NTG 注射大鼠受损的代谢途径。

方法

雄性 Wistar 大鼠（N=40）分为两组，分别接受 10%FS 或自来水。45 天后，将它们分为 NTG 注射（10mg/kg；15 天）或对照组。第四次 NTG 注射后，进行 F-氟脱氧葡萄糖（[F] FDG）微 PET 扫描。注射后的第二天，进行安乐死，并采集血液以进行血糖和 LDH 分析。评估大脑皮质中的能量分子、TBARS、PGC-1α 和 MCTS1 水平。评估三叉神经节（TG）中激活的卫星神经胶质细胞（SGC）。

主要发现

血糖或 LDH 血清水平没有变化。与 NTG 无注射组相比，NTG 注射大鼠下丘脑（HT）的葡萄糖摄取显著增加。FS-NTG 组与 NTG 组相比，上丘（SC）的代谢增加。此外，与 FS 组相比，FS-NTG 组的下丘（IC）葡萄糖摄取增加。FS-NTG 大鼠皮质肌苷水平明显高于 NTG 或 FS 组，TBARS 或 MCTS1 水平无变化，尽管 FS+NTG 组中 PGC1-α 含量略有下降。最后，FS-NTG 大鼠 TG 周围的激活 SGC 明显增加。