School of Life Sciences, Hefei Normal University, Hefei 230601, China.
School of Basic Medicine, Bengbu Medical College, Bengbu 233030, China.
Int J Biol Macromol. 2023 Aug 1;245:125538. doi: 10.1016/j.ijbiomac.2023.125538. Epub 2023 Jun 22.
Polysaccharides from Armillariella tabescens mycelia (AT) have a potent anti-inflammatory effect. Nevertheless, the impact of AT on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) has not yet been illustrated. This study aimed to explore AT's impact on experimental UC mice and investigate its underlying mechanisms. Mice were given DSS, 0.2 g/kg AT or 0.4 g/kg AT for seven days, and the symptoms of UC were observed. The serum and colon samples were harvested to analyze the biochemical indices and inflammasome-related proteins. The feces were collected to analyze short-chain fatty acids (SCFAs) and gut microbiota. The present study found that AT improved the symptoms of UC, colonic oxidative stress, and inflammation. AT treatment elevated SCFAs contents and colonic barrier function. Furthermore, AT improved microbial community structure through the selective enrichment of beneficial bacterial species. In conclusion, these results underlined that AT improved DSS-induced colitis and inhibited colonic inflammation via regulating the intestinal microbial community and enhancing the colonic mucosal barrier.
银耳菌丝体多糖（AT）具有很强的抗炎作用。然而，AT 对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）的影响尚未阐明。本研究旨在探讨 AT 对实验性 UC 小鼠的影响，并研究其潜在机制。小鼠给予 DSS、0.2 g/kg AT 或 0.4 g/kg AT 连续 7 天，观察 UC 症状。采集血清和结肠样本，分析生化指标和炎症小体相关蛋白。收集粪便，分析短链脂肪酸（SCFAs）和肠道微生物群。本研究发现 AT 改善了 UC 的症状、结肠氧化应激和炎症。AT 治疗增加了 SCFAs 含量和结肠屏障功能。此外，AT 通过选择性富集有益细菌种类来改善微生物群落结构。总之，这些结果表明，AT 通过调节肠道微生物群落和增强结肠黏膜屏障，改善 DSS 诱导的结肠炎并抑制结肠炎症。
