DevR 磷酸化在链霉菌 A3(2) M145 一氧化氮动态平衡和信号传导中的作用。
Role of DevR phosphorylation in nitric oxide homeostasis and signaling of Streptomyces coelicolor A3(2) M145.
机构信息
Faculty of Life Sciences, Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 1568502, Japan.
出版信息
FEMS Microbiol Lett. 2023 Jan 17;370. doi: 10.1093/femsle/fnad060.
Our previous studies revealed that a two-component system (TCS), DevS, and DevR, regulate both nitric oxide (NO) signaling and NO homeostasis in the actinobacterium Streptomyces coelicolor A3(2) M145, suggesting a reasonable system for NO-dependent metabolism. In this study, sequence alignment of DevR and DevR homologs found Asp66 (D66) and Thr196 (T196) as predicted phosphorylation sites of DevR. Phos-tag gel electrophoretic mobility shift assay suggested that D66 and T196 are involved in the phosphorylation of DevR. The respective point mutations of D66 and T196 significantly decreased the transcriptional activity of DevR, which affected nitrite production and aerial mycelium formation. These results suggested that both D66 and T196 of DevR are important for the regulation of NO homeostasis and signaling in S. coelicolor A3(2) M145.
我们之前的研究表明，双组分系统 (TCS) DevS 和 DevR 调节链霉菌 A3(2) M145 中的一氧化氮 (NO) 信号和 NO 动态平衡，这表明该系统可用于依赖 NO 的代谢。在这项研究中，对 DevR 和 DevR 同源物的序列比对发现，Asp66 (D66) 和 Thr196 (T196) 是 DevR 的预测磷酸化位点。Phos-tag 凝胶电泳迁移率变动分析表明，D66 和 T196 参与了 DevR 的磷酸化。D66 和 T196 的相应点突变显著降低了 DevR 的转录活性，从而影响了亚硝酸盐的产生和气生菌丝的形成。这些结果表明，DevR 的 D66 和 T196 对 S. coelicolor A3(2) M145 中 NO 动态平衡和信号转导的调节都很重要。
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