Taubenschmid-Stowers Jasmin, Reik Wolf
Altos Labs Cambridge Institute of Science, Cambridge, UK.
Altos Labs Cambridge Institute of Science, Cambridge, UK; Babraham Institute, Cambridge, UK; Wellcome Sanger Institute, Cambridge, UK; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK. Electronic address: https://twitter.com/@ReikLab.
Curr Opin Genet Dev. 2023 Aug;81:102066. doi: 10.1016/j.gde.2023.102066. Epub 2023 Jun 23.
The totipotent embryo initiates transcription during zygotic or embryonic genome activation (EGA, ZGA). ZGA occurs at the 8-cell stage in humans and its failure leads to developmental arrest. Understanding the molecular pathways underlying ZGA and totipotency is essential to comprehend human development. Recently, human 8-cell-like cells (8CLCs) have been discovered in vitro that resemble the 8-cell embryo. 8CLCs exist among naive pluripotent stem cells and can be induced genetically or chemically. Their ZGA-like transcriptome, transposable element activation, 8-cell embryo-specific protein expression, and developmental properties make them an exceptional model system to study early embryonic cell-state transitions and human totipotency programs in vitro.
全能胚胎在合子或胚胎基因组激活（EGA，ZGA）期间启动转录。ZGA在人类的8细胞阶段发生，其失败会导致发育停滞。了解ZGA和全能性背后的分子途径对于理解人类发育至关重要。最近，在体外发现了类似于8细胞胚胎的人类8细胞样细胞（8CLC）。8CLC存在于原始多能干细胞中，可以通过基因或化学方法诱导产生。它们类似ZGA的转录组、转座元件激活、8细胞胚胎特异性蛋白表达以及发育特性，使其成为体外研究早期胚胎细胞状态转变和人类全能性程序的特殊模型系统。
