Neurosciences, Pharmacology and Environment Team, Laboratory of Clinical, Experimental and Environmental Neurosciences, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco.
Neurosciences, Pharmacology and Environment Team, Laboratory of Clinical, Experimental and Environmental Neurosciences, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco; Biology, Health and Ecology Laboratory, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, Tetouan, Morocco.
Food Chem Toxicol. 2023 Aug;178:113904. doi: 10.1016/j.fct.2023.113904. Epub 2023 Jun 23.
Parkinson's disease (PD) is a progressive neurodegenerative disease characterized by the degeneration of substantia nigra dopaminergic neurons. Many therapeutic strategies were explored for PD with no success. In this study, we investigated the efficacy of graphene oxide nanoparticles (GONPs) using the reserpine model of PD. Low concentrations GONPs were utilized as a therapeutic agent in many neurodegenerative diseases. We assessed the neurobehavioral alterations in the reserpine model of PD and investigated the neuroprotective and antioxidant effects of GONPs in this model. Thirty male mice were separated into three groups (N = 10): C (control); Res (Reserpine 0.25 mg/kg); Res + GONPs (Reserpine 0.25 mg/kg and GONPs 25 mg/kg). Our results showed that reserpine neurotoxicity induced hypoactivity with a significant increase of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) levels in the brain and brainstem. GONPs reversed the reserpine-induced hypoactivity concomitant with decreased neuronal CAT and MDA levels. These findings support the potential use of GONPs as an antioxidant agent in the central nervous system (CNS) that protects against neurodegeneration in the reserpine PD model.
帕金森病（PD）是一种进行性神经退行性疾病，其特征是黑质多巴胺能神经元的退化。许多治疗策略都被探索用于 PD，但均未成功。在这项研究中，我们使用利血平 PD 模型研究了氧化石墨烯纳米粒子（GONPs）的疗效。低浓度的 GONPs 已被用作许多神经退行性疾病的治疗剂。我们评估了 PD 利血平模型中的神经行为改变，并研究了 GONPs 在该模型中的神经保护和抗氧化作用。30 只雄性小鼠被分为三组（N=10）：C（对照）；Res（利血平 0.25mg/kg）；Res+GONPs（利血平 0.25mg/kg 和 GONPs 25mg/kg）。我们的结果表明，利血平神经毒性诱导活动减少，大脑和脑干中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）水平显著升高。GONPs 逆转了利血平诱导的活动减少，同时降低了神经元 CAT 和 MDA 水平。这些发现支持将 GONPs 用作中枢神经系统（CNS）中的抗氧化剂，以防止利血平 PD 模型中的神经退行性变。
