School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
Department of Pharmacy, Sichuan Orthopedic Hospital, Chengdu, China.
J Ethnopharmacol. 2023 Dec 5;317:116846. doi: 10.1016/j.jep.2023.116846. Epub 2023 Jun 24.
Tou Nong Powder (TNP), a classical Chinese medicinal formula originated from the Chinese Ming Dynasty, has been applied to treat skin ulcers in patients with deficient constitutions. According to theory of traditional Chinese medicine, colonic ulcers share similar pathological conditions with skin ulcers, and consequently, TNP has been applied to ulcerative colitis (UC) safely and effectively.
To investigate whether TNP obstructs 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced enteric inflammation through regulation of NLRP3 inflammasome and attenuating enteric pyroptosis.
Network pharmacology and UPLC-Q-TOF/MS were operated to identify compounds and pharmacological potential targets. The therapeutic effects of TNP were assessed on TNBS induced colitis via general symptoms (disease activity index, colonic weight and length) and histopathological observation. The NF-κB/NLRP3/Caspase-1/GSDMD signaling pathway regulation was investigated by Western blot and real time reverse transcription polymerase chain reaction (RT-qPCR).
TNP ameliorates the disease activity index, reverses the increase of colonic weight increase, alleviates colonic shortening and colonic histopathological injury. A decrease in tumor necrosis factor α (TNF-α), diamine oxidase (DAO), intercellular adhesion molecule-1 (ICAM-1), and endo-toxin (ET) were investigated in peripheral circulation. Moreover, TNP significantly obstructed the NF-κB/NLRP3/Caspase-1/GSDMD signaling pathway.
TNP displays a promising therapeutic effect on UC via suppressing NF-κB/NLRP3/Caspase-1/GSDMD signaling pathway and reducing the expression of IL-1β and IL-18.
痛宁散（TNP）是一种源自中国明朝的经典中药方剂，已被用于治疗体质虚弱患者的皮肤溃疡。根据中医理论，结肠溃疡与皮肤溃疡具有相似的病理条件，因此 TNP 已被安全有效地应用于溃疡性结肠炎（UC）。
研究 TNP 是否通过调节 NLRP3 炎性体和减轻肠道细胞焦亡来抑制 2,4,6-三硝基苯磺酸（TNBS）诱导的肠道炎症。
运用网络药理学和 UPLC-Q-TOF/MS 鉴定化合物和药理学靶标。通过一般症状（疾病活动指数、结肠重量和长度）和组织病理学观察评估 TNP 对 TNBS 诱导的结肠炎的治疗作用。通过 Western blot 和实时逆转录聚合酶链反应（RT-qPCR）研究 NF-κB/NLRP3/Caspase-1/GSDMD 信号通路的调节。
TNP 改善了疾病活动指数，逆转了结肠重量增加，减轻了结肠缩短和结肠组织病理学损伤。在外周循环中，肿瘤坏死因子-α（TNF-α）、二胺氧化酶（DAO）、细胞间黏附分子-1（ICAM-1）和内毒素（ET）的水平下降。此外，TNP 显著抑制了 NF-κB/NLRP3/Caspase-1/GSDMD 信号通路。
TNP 通过抑制 NF-κB/NLRP3/Caspase-1/GSDMD 信号通路和降低 IL-1β和 IL-18 的表达，显示出对 UC 有良好的治疗效果。
