Yoshioka T, Nagami K, Tamaki T, Nakano S
Jpn J Physiol. 1986;36(2):379-90. doi: 10.2170/jjphysiol.36.379.
The contractility of detergent-treated frog skeletal muscle and its ultrastructure were studied. Triton X-100 and Brij-58 were used as nonionic surface active substances. The concentrations of the detergent solutions were adjusted so that the trains of the twitch tensions would continue for about 10 min. The duration of the twitch tensions was about 10 min when the muscles were soaked in 0.1 mM (0.006%) Triton or in 2.5 mM (0.280%) Brij solution. The twitch tensions did not recover upon returning the muscles to the normal Ringer solution after treatment of the detergents. Rapid cooling of muscle that had been soaked in a 1.0 mM caffeine Ringer solution for 10 min provoked a marked contracture (RCC) in 0.1-0.2 mM Triton-treated preparation. No such contracture was obtained in 1.0-2.5 mM Brij-treated preparation. In ultrastructural findings of the Triton-treated muscle, some structural changes were observed in the transverse tubule and the junctional gap, but not in the terminal cisternae. Brij-treated muscle showed damage of each of these membranes, including the terminal cisternae. These findings suggest that the two detergents have different surface activities on the internal membrane system of the skeletal muscle. The biological activities of the membrane remained even after the treatment of Triton with a suitable concentration.
研究了经去污剂处理的青蛙骨骼肌的收缩性及其超微结构。使用 Triton X - 100 和 Brij - 58 作为非离子表面活性物质。调整去污剂溶液的浓度，使单收缩张力序列持续约 10 分钟。当肌肉浸泡在 0.1 mM（0.006%）的 Triton 或 2.5 mM（0.280%）的 Brij 溶液中时，单收缩张力的持续时间约为 10 分钟。在用去污剂处理后将肌肉放回正常林格液中，单收缩张力未恢复。在浸泡于 1.0 mM 咖啡因林格液 10 分钟的肌肉快速冷却后，0.1 - 0.2 mM Triton 处理的标本中出现明显的挛缩（RCC）。在 1.0 - 2.5 mM Brij 处理的标本中未获得这种挛缩。在 Triton 处理的肌肉的超微结构观察中，在横小管和连接间隙观察到一些结构变化，但在终池未观察到。Brij 处理的肌肉显示这些膜中的每一个都有损伤，包括终池。这些发现表明这两种去污剂对骨骼肌内膜系统具有不同的表面活性。在用合适浓度的 Triton 处理后，膜的生物学活性仍然存在。
