Departamento de Investigaciones en Polímeros y Materiales, Universidad de Sonora, Hermosillo, Sonora, 83000, Mexico.
Departamento de Medicina y Ciencias de la Salud, Universidad de Sonora, Hermosillo, 83000, Mexico.
Chem Biodivers. 2023 Jul;20(7):e202300051. doi: 10.1002/cbdv.202300051. Epub 2023 Jun 26.
Acute monocytic leukemia is a type of myeloid leukemia that develops in monocytes. The current clinical therapies for leukemia are unsatisfactory due to their side effects and nonspecificity toward target cells. Some lectins display antitumor activity and may specifically recognize cancer cells by binding to carbohydrate structures on their surface. Therefore, this study evaluated the response of the human monocytic leukemia cell lines THP-1 to the Olneya tesota PF2 lectin. The induction of apoptosis and reactive oxygen species production in PF2-treated cells was evaluated by flow cytometry, and the lectin-THP-1 cell interaction and mitochondrial membrane potential were evaluated by confocal fluorescence microscopy. PF2 genotoxicity was evaluated by DNA fragmentation analysis via gel electrophoresis. The results showed that PF2 binds to THP-1 cells, triggers apoptosis and DNA degradation, changes the mitochondrial membrane potential, and increases reactive oxygen species levels in PF2-treated THP-1 cells. These results suggest the potential use of PF2 for developing alternative anticancer treatments with enhanced specificity.
急性单核细胞白血病是一种起源于单核细胞的髓系白血病。由于其副作用和对靶细胞的非特异性，目前白血病的临床治疗方法并不令人满意。一些凝集素具有抗肿瘤活性，可能通过与细胞表面的碳水化合物结构结合特异性识别癌细胞。因此，本研究评估了人类单核细胞白血病细胞系 THP-1 对 Olneya tesota PF2 凝集素的反应。通过流式细胞术评估 PF2 处理细胞中细胞凋亡和活性氧产生的诱导，通过共聚焦荧光显微镜评估凝集素-THP-1 细胞相互作用和线粒体膜电位。通过凝胶电泳的 DNA 片段分析评估 PF2 的遗传毒性。结果表明，PF2 与 THP-1 细胞结合，触发细胞凋亡和 DNA 降解，改变线粒体膜电位，并增加 PF2 处理的 THP-1 细胞中活性氧的水平。这些结果表明 PF2 可能被用于开发具有增强特异性的新型抗癌治疗方法。
