一种用于大量纯化胎牛血清乙酰胆碱酯酶的简化程序。
A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase.
作者信息
De la Hoz D, Doctor B P, Ralston J S, Rush R S, Wolfe A D
出版信息
Life Sci. 1986 Jul 21;39(3):195-9. doi: 10.1016/0024-3205(86)90530-8.
PMID:3736320
Abstract
A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure.
摘要
已经开发出一种用于大规模纯化胎牛血清乙酰胆碱酯酶（AChE）（EC 3.1.1.7）的简单方法。该方法包括两个步骤：将250升血清中的AChE分批吸附到普鲁卡因酰胺亲和琼脂糖4B凝胶上；以及对第一步产物进行分析性普鲁卡因酰胺亲和色谱法。用此方法在10天内纯化出了超过100毫克的AChE，达到了表观均一性。
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