College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, PR China.
College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5;302:123065. doi: 10.1016/j.saa.2023.123065. Epub 2023 Jun 21.
Lipopolysaccharide (LPS) as the component of cell membrane on gram-negative bacteria played a central role on inflammatory inducer to stimulate a multi-system host response. Herein, a surface-enhanced fluorescent (SEF) sensor was developed for LPS analysis based on shell-isolated nanoparticles (SHINs). The fluorescent signal of CdTe quantum dots (QDs) was amplified by silica shell-coated Au nanoparticles (Au NPs). The 3D finite-difference time-domain (3D-FDTD) simulation revealed that this enhancement was due to local electric field amplification. This method has a linear detection range of 0.1-20 μg/mL and a detection limit of 64 ng/mL for LPS. Furthermore, the developed method was successfully applied for LPS analysis in milk and human serum sample. The results indicated that the as-prepared sensor has significant potential for selective detection of LPS in biomedical diagnosis and food safety.
脂多糖（LPS）作为革兰氏阴性菌细胞膜的组成部分，在炎症诱导物中起着核心作用，能刺激多系统宿主反应。在此，基于壳层分离纳米粒子（SHINs），我们开发了一种用于 LPS 分析的表面增强荧光（SEF）传感器。CdTe 量子点（QDs）的荧光信号被涂有二氧化硅壳的金纳米粒子（Au NPs）放大。3D 有限差分时域（3D-FDTD）模拟表明，这种增强是由于局部电场的放大。该方法对 LPS 的线性检测范围为 0.1-20μg/mL，检测限为 64ng/mL。此外，该方法还成功地应用于牛奶和人血清样品中 LPS 的分析。结果表明，所制备的传感器在生物医学诊断和食品安全中对 LPS 的选择性检测具有重要的应用潜力。
