Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
Bioresour Technol. 2023 Oct;385:129385. doi: 10.1016/j.biortech.2023.129385. Epub 2023 Jun 25.
The effect of hydraulic retention time (HRT) on the continuous lactate-driven dark fermentation (LD-DF) of food waste (FW) was investigated. The robustness of the bioprocess against feast/famine perturbations was also explored. The stepwise HRT decrease from 24 to 16 and 12 h in a continuously stirred tank fermenter fed with simulated restaurant FW impacted on hydrogen production rate (HPR). The optimal HRT of 16 h supported a HPR of 4.2 L H/L-d. Feast/famine perturbations caused by 12-h feeding interruptions led to a remarkable peak in HPR up to 19.2 L H/L-d, albeit the process became stable at 4.3 L H/L-d following perturbation. The occurrence of LD-DF throughout the operation was endorsed by metabolites analysis. Particularly, hydrogen production correlated positively with lactate consumption and butyrate production. Overall, the FW LD-DF process was highly sensitive but resilient against transient feast/famine perturbations, supporting high-rate HPRs under optimal HRTs.
考察了水力停留时间 (HRT) 对食品废物 (FW) 连续乳酸驱动暗发酵 (LD-DF) 的影响。还探索了生物工艺对丰/饥荒扰动的鲁棒性。在连续搅拌罐式发酵器中，逐步将 HRT 从 24 小时减少到 16 小时和 12 小时，进料模拟餐厅 FW 会影响氢气产生率 (HPR)。16 小时的最佳 HRT 支持 4.2 L H/L-d 的 HPR。由于 12 小时的进料中断导致的丰/饥荒扰动导致 HPR 显著峰值达到 19.2 L H/L-d，但扰动后该过程稳定在 4.3 L H/L-d。通过代谢物分析证实了整个操作过程中的 LD-DF。特别是，氢气的产生与乳酸的消耗和丁酸的产生呈正相关。总的来说，FW LD-DF 工艺对瞬时丰/饥荒扰动非常敏感，但具有弹性，在最佳 HRT 下支持高 HPR。
