Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain.
Microbiology Department of the University Hospital of Burgos (HUBU), Burgos, Spain.
Foodborne Pathog Dis. 2023 Aug;20(8):368-373. doi: 10.1089/fpd.2023.0010. Epub 2023 Jun 27.
spp. is the leading cause of foodborne gastrointestinal infections in humans worldwide. This study reports the first case of four family members who had contact with the same source of contamination with different results. Only the little siblings were infected by the same strain, but with different symptoms. Whereas the daughter was slightly affected with mild enteritis, the son suffered a longer campylobacteriosis followed with a perimyocarditis. This is the first case of the youngest patient affected by -related perimyocarditis published to date. The genomes of both strains were characterized by whole-genome sequencing and compared with the NCTC 11168 genome to gain insights into the molecular features that may be associated with perimyocarditis. Various comparison tools were used for the comparative genomics analysis, including the identification of virulence and antimicrobial resistance genes, phase variable (PV) genes, and single nucleotide polymorphisms (SNPs) identification. Comparisons of the strains identified 16 SNPs between them, which constituted small but significant changes mainly affecting the ON/OFF state of PV genes after passing through both hosts. These results suggest that PV occurs during human colonization, which modulates bacteria virulence through human host adaptation, which ultimately is related to complications after a campylobacteriosis episode depending on the host status. The findings highlight the importance of the relation between host and pathogen in severe complications of infections.
spp. 是全球导致食源性胃肠道感染的主要原因。本研究报告了首例 4 名家庭成员接触同一污染来源但结果不同的病例。只有兄弟姐妹被同一菌株感染，但症状不同。女儿受轻微肠炎影响，儿子受较长时间的弯曲菌病和心肌炎影响。这是迄今为止首例受感染相关心肌炎影响的最小患者。通过全基因组测序对两种菌株的基因组进行了表征，并与 NCTC 11168 基因组进行了比较，以深入了解可能与心肌炎相关的分子特征。使用各种比较工具进行了比较基因组学分析，包括毒力和抗微生物药物耐药性基因、相变异（PV）基因和单核苷酸多态性（SNP）的鉴定。对菌株的比较鉴定了它们之间的 16 个 SNP，这些 SNP 虽然很小，但变化显著，主要影响通过两个宿主后 PV 基因的 ON/OFF 状态。这些结果表明，PV 发生在人类定植期间，通过人类宿主适应调节细菌的毒力，最终根据宿主状态与弯曲菌病发作后的并发症有关。研究结果强调了宿主和病原体之间关系在 感染严重并发症中的重要性。
