College of Life Sciences, Chongqing Normal University, Chongqing 401331, China.
Biosensors (Basel). 2023 Jun 3;13(6):613. doi: 10.3390/bios13060613.
The monitoring of sulfadiazine (SDZ) is of great significance for food safety, environmental protection, and human health. In this study, a fluorescent aptasensor based on MnO and FAM-labeled SDZ aptamer (FAM-SDZ30-1) was developed for the sensitive and selective detection of SDZ in food and environmental samples. MnO nanosheets adsorbed rapidly to the aptamer through its electrostatic interaction with the base, providing the basis for an ultrasensitive SDZ detection. Molecular dynamics was used to explain the combination of SMZ1S and SMZ. This fluorescent aptasensor exhibited high sensitivity and selectivity with a limit of detection of 3.25 ng/mL and a linear range of 5-40 ng/mL. The recoveries ranged from 87.19% to 109.26% and the coefficients of variation ranged from 3.13% to 13.14%. In addition, the results of the aptasensor showed an excellent correlation with high-performance liquid chromatography (HPLC). Therefore, this aptasensor based on MnO is a potentially useful methodology for highly sensitive and selective detection of SDZ in foods and environments.
磺胺嘧啶（SDZ）的监测对于食品安全、环境保护和人类健康具有重要意义。在本研究中，开发了一种基于 MnO 和 FAM 标记的磺胺嘧啶适配体（FAM-SDZ30-1）的荧光适体传感器，用于灵敏和选择性地检测食品和环境样品中的 SDZ。MnO 纳米片通过与碱基的静电相互作用快速吸附到适配体上，为超灵敏的 SDZ 检测提供了基础。分子动力学用于解释 SMZ1S 和 SMZ 的结合。这种荧光适体传感器具有高灵敏度和选择性，检测限为 3.25ng/mL，线性范围为 5-40ng/mL。回收率范围为 87.19%至 109.26%，变异系数范围为 3.13%至 13.14%。此外，适体传感器的结果与高效液相色谱（HPLC）具有极好的相关性。因此，基于 MnO 的这种适体传感器为食品和环境中 SDZ 的高灵敏和选择性检测提供了一种潜在有用的方法。
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