College of Life Sciences, Chongqing Normal University, Chongqing 401331, PR China.
College of Life Sciences, Chongqing Normal University, Chongqing 401331, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 5;304:123290. doi: 10.1016/j.saa.2023.123290. Epub 2023 Aug 23.
Sulfamethazine (SMZ) has been widely used in animal husbandry and exposed to water and soil environments, posing potential threat to human health and ecological environment. Hence, we designed a CoOOH-based aptasensor, the fluorescence resonance energy transfer between FAM-labeled aptamer and CoOOH was used to sensitively and selectively detect SMZ in water and soil environments. Molecular docking and molecular dynamics simulations were used to predict binding mechanisms of SMZ and aptamer. Under optimized conditions, the aptasensor exhibited high sensitivity and selectivity with a linear range of 5-40 ng/mL and a limit of detection of 2.43 ng/mL. The recoveries of the aptasensor were 84.6-115.8% in water and soil samples with relative standard deviations below 9%, and the detection results were highly consistent with high-performance liquid chromatography. Therefore, this developed aptasensor was a reliable tool and could be applied to monitoring of SMZ in environmental samples.
磺胺甲恶唑（SMZ）在畜牧业中被广泛应用，且会暴露于水和土壤环境中，对人类健康和生态环境构成潜在威胁。因此，我们设计了一种基于 CoOOH 的适体传感器，利用 FAM 标记的适体与 CoOOH 之间的荧光共振能量转移来灵敏且选择性地检测水和土壤环境中的 SMZ。通过分子对接和分子动力学模拟预测了 SMZ 和适体的结合机制。在优化条件下，该适体传感器具有较高的灵敏度和选择性，线性范围为 5-40ng/mL，检测限为 2.43ng/mL。该适体传感器在水和土壤样品中的回收率为 84.6-115.8%，相对标准偏差低于 9%，检测结果与高效液相色谱高度一致。因此，该开发的适体传感器是一种可靠的工具，可用于环境样品中 SMZ 的监测。
