Advanced Science Research Laboratory, Saitama Institute of Technology, 1690, Fusaiji, Fukaya, Saitama 369-0293, Japan.
Department of Life Science and Green Chemistry, Graduate School of Engineering, Saitama Institute of Technology, 1690, Fusaiji, Fukaya, Saitama 369-0293, Japan.
Biosensors (Basel). 2023 Jun 9;13(6):638. doi: 10.3390/bios13060638.
Polyion complex (PIC) materials have been widely used in biosensors due to their molecular selectivity. However, achieving both widely controllable molecular selectivity and long-term solution stability with traditional PIC materials has been challenging due to the different molecular structures of polycations (poly-C) and polyanions (poly-A). To address this issue, we propose a novel polyurethane (PU)-based PIC material in which the main chains of both poly-A and poly-C are composed of PU structures. In this study, we electrochemically detect dopamine (DA) as the analyte and L-ascorbic acid (AA) and uric acid (UA) as the interferents to evaluate the selective property of our material. The results show that AA and UA are significantly eliminated, while DA can be detected with a high sensitivity and selectivity. Moreover, we successfully tune the sensitivity and selectivity by changing the poly-A and poly-C ratios and adding nonionic polyurethane. These excellent results were employed in the development of a highly selective DA biosensor with a detection range from 500 nM to 100 μM and a 3.4 μM detection limit. Overall, our novel PIC-modified electrode has the potential to advance biosensing technologies for molecular detection.
聚离子复合物（PIC）材料由于其分子选择性而被广泛应用于生物传感器中。然而，由于聚阳离子（poly-C）和聚阴离子（poly-A）的分子结构不同，传统的 PIC 材料很难同时实现广泛可控的分子选择性和长期溶液稳定性。为了解决这个问题，我们提出了一种新型的基于聚氨酯（PU）的 PIC 材料，其中聚 A 和聚 C 的主链都由 PU 结构组成。在这项研究中，我们通过电化学检测多巴胺（DA）作为分析物，以及 L-抗坏血酸（AA）和尿酸（UA）作为干扰物，来评估我们材料的选择性。结果表明，AA 和 UA 被显著消除，而 DA 可以具有高灵敏度和选择性地检测到。此外，我们还通过改变聚 A 和聚 C 的比例以及添加非离子型聚氨酯成功地调节了灵敏度和选择性。这些优异的结果被应用于开发一种具有高选择性的 DA 生物传感器，其检测范围为 500 nM 至 100 μM，检测限为 3.4 μM。总的来说，我们的新型 PIC 修饰电极有可能推动用于分子检测的生物传感技术的发展。
