Carvalho Thiele da Silva, Silva Carlos Alberto Domingues da, Martins Celso Feitosa, Silva Laryssa Lemos da, Zanuncio José Cola, Serrão José Eduardo
Programa de Pós-Graduação em Zoologia, Universidade Federal da Paraíba, Campus I, Castelo Branco, João Pessoa 58059-900, Brazil.
Laboratório de Entomologia, Embrapa Algodão, Rua Oswaldo Cruz, 1143, Campina Grande 58428-095, Brazil.
Insects. 2023 Jun 20;14(6):571. doi: 10.3390/insects14060571.
Boheman (Coleoptera: Curculionidae) survives on alternative diets; however, this induces reproductive diapause. The objective was to evaluate the morphology and morphometry of the reproductive tract of this weevil after feeding on alternative diets. The experimental design was completely randomized with 160 replications and treatments arranged in a factorial design 3 × 3, represented by adults fed on 3 food types (fragments of banana (T1) or orange (T2) endocarp, or with cotton squares of cultivar BRS 286 (T3, control)) and three evaluation periods (30, 60, and 90 days) and after each of these periods they were fed with cotton squares for 10 days. The reproductive tract of 100% of females fed banana endocarp, orange endocarp, and cotton squares for 30 and 60 days and then cotton squares were morphologically adequate for reproduction, and after 90 days, only 50% of those fed cotton squares were in this condition. The length of the ovarioles and the width of the mature oocyte were greater for fed on cotton squares and smaller in those with banana and orange endocarps. Histological sections reveal that male testes even with strong degenerative signals are already producing spermatozoa. On the other hand, females displayed ovaries with nurse cells in the tropharium and some maturing oocytes in the vitellarium. The body length was longer and the testis area and diameter smaller in males fed on cotton squares than in those with banana and orange endocarp. females fed for ≥90 days with alternative food sources do not recover the functionality of their reproductive tract, even after consuming, for 10 days, a diet that favors reproduction. On the other hand, the males remain with their reproductive organs functional with this condition.
博赫曼象甲（鞘翅目：象甲科）能以替代食物为生；然而，这会导致生殖滞育。目的是评估这种象甲取食替代食物后其生殖道的形态和形态测量学特征。实验设计为完全随机，有160次重复，处理采用3×3析因设计，由取食3种食物类型（香蕉（T1）或橙子（T2）内果皮碎片，或品种为BRS 286的棉方块（T3，对照））的成虫以及3个评估期（30天、60天和90天）组成，在每个评估期后，它们以棉方块为食10天。取食香蕉内果皮、橙子内果皮和棉方块30天和60天，然后再取食棉方块的100%雌性象甲的生殖道在形态上适合繁殖，而在90天后，只有50%取食棉方块的象甲处于这种状态。取食棉方块的象甲卵巢小管长度和成熟卵母细胞宽度更大，而取食香蕉和橙子内果皮的象甲则较小。组织学切片显示，雄性睾丸即使有强烈的退化信号仍在产生精子。另一方面，雌性象甲的卵巢在滋养区有营养细胞，在卵黄生成区有一些成熟的卵母细胞。取食棉方块的雄性象甲体长更长，睾丸面积和直径比取食香蕉和橙子内果皮的雄性象甲小。取食替代食物源≥90天的雌性象甲即使在食用有利于繁殖的食物10天后，其生殖道功能也无法恢复。另一方面，在这种情况下雄性象甲的生殖器官仍保持功能。
