Balbierz H, Nikołajczuk M
Arch Immunol Ther Exp (Warsz). 1978;26(1-6):231-2.
CEA antigen obtained by the Wrocław team and CEA standard antigen from Chester Beatty Cancer Research Institute, London were used for immunization of goats. Two immunization methods were adopted: one with decreasing antigen doses beginning from 500 microgram and the other with equal, small doses of 10 microgram. Anti-goat IgG1 + IgG2 immune serum was obtained by immunizing a horse with 10 mg of the antigen with complete Freund's adjuvant. Anti-CEA immune sera obtained after immunization with small doses were highly active; in the RIA test they showed half maximum CEA binding at 1:40,000 and 1:80,000 dilutions. Likewise, the anti IgG1 and IgG2 serum from the horse proved to be highly active.
弗罗茨瓦夫团队获得的癌胚抗原（CEA）以及来自伦敦切斯特·比蒂癌症研究所的CEA标准抗原被用于山羊免疫。采用了两种免疫方法：一种是从500微克开始递减抗原剂量，另一种是采用10微克的等量小剂量。用10毫克抗原加完全弗氏佐剂免疫一匹马，获得了抗山羊IgG1 + IgG2免疫血清。小剂量免疫后获得的抗CEA免疫血清活性很高；在放射免疫分析（RIA）测试中，它们在1:40,000和1:80,000稀释度时显示出半数最大CEA结合。同样，来自马的抗IgG1和IgG2血清也被证明具有高活性。
