Department of Hematology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
Department of Hematology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China.
Hematology. 2023 Dec;28(1):2227491. doi: 10.1080/16078454.2023.2227491.
Necroptosis has been reported to play an important role in different cancers, including leukemia. However, biomarkers of necroptosis-related genes (NRGs) that help predict the prognosis of AML are still lacking. Our research aims to build a novel signature of NRGs that could enhance our understanding of the molecular heterogeneity in leukemia.
Gene expression profiles as well as clinical features were downloaded from TCGA and GEO databases. Data analysis were executed using R software version 4.2.1 and GraphPad Prism version 9.0.0.
Univariate cox regression and lasso regression were applied to identify survival-specific genes. Four genes including FADD, PLA2G4A, PYCARD and ZBP1 were considered as independent risk factors that affect the prognosis of patients. Risk scores were calculated according to the coefficient of four genes. Then clinical characteristics and risk scores were enrolled to construct a nomogram. CellMiner was also used to screen potential drugs and analyze the correlations between genes and drug sensitivity.
In general, we established a signature of four genes related to necroptosis that could be helpful for future risk stratification in patients with AML.
已报道细胞坏死性凋亡（Necroptosis）在包括白血病在内的多种癌症中发挥重要作用。然而，缺乏有助于预测 AML 预后的细胞坏死性凋亡相关基因（NRGs）生物标志物。本研究旨在构建 NRGs 的新型标志物，以增强我们对白血病分子异质性的理解。
从 TCGA 和 GEO 数据库中下载基因表达谱和临床特征。使用 R 软件版本 4.2.1 和 GraphPad Prism 版本 9.0.0 进行数据分析。
单因素 cox 回归和lasso 回归用于鉴定与生存相关的基因。FADD、PLA2G4A、PYCARD 和 ZBP1 这四个基因被认为是影响患者预后的独立危险因素。根据四个基因的系数计算风险评分。然后将临床特征和风险评分纳入构建列线图。还使用 CellMiner 筛选潜在药物并分析基因与药物敏感性之间的相关性。
总的来说，我们建立了一个与细胞坏死性凋亡相关的四个基因的标志物，有助于未来对 AML 患者进行风险分层。
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