Chuppina L M, Vlasova I G
Neurosci Behav Physiol. 1986 Jan-Feb;16(1):17-22. doi: 10.1007/BF01186884.
The process of differentiation of neurons was traced on an organotypic cerebellar culture of newborn mice. Cerebellar cells reach morphological maturity by the 18-21st day of culturing. An increase of the membrane potential begins on the 6-7th day of culturing. It reaches values characteristic for the definitive stage of the neuron (65-75 mV) by the 9-10th day of culturing. Spontaneous action potentials begin to be recorded on the 10-12th day of culturing. At this time they markedly differ in their characteristics from the action potential of the mature neuron. The differences become less noticeable by the 16-18th day of culturing. However, final maturation of the action potential occurs at later times. The formation of specific sensitivity of cerebellar neurons to acetylcholine correlates with the time of formation of the action potential.
在新生小鼠的小脑器官型培养物上追踪神经元的分化过程。小脑细胞在培养的第18 - 21天达到形态成熟。膜电位的增加在培养的第6 - 7天开始。到培养的第9 - 10天，它达到神经元最终阶段的特征值（65 - 75 mV）。在培养的第10 - 12天开始记录到自发动作电位。此时，它们的特征与成熟神经元的动作电位明显不同。到培养的第16 - 18天，这些差异变得不那么明显。然而，动作电位的最终成熟发生在更晚的时候。小脑神经元对乙酰胆碱的特异性敏感性的形成与动作电位的形成时间相关。
