Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Clinical Research Development Unit, Shahid Mostafa Khomeini Hospital, Ilam University of Medical Science, Ilam, Iran.
Cell Biol Int. 2023 Oct;47(10):1728-1736. doi: 10.1002/cbin.12064. Epub 2023 Jun 27.
Several studies have revealed that vitamin D deficiency is linked to an increased risk of developing coronavirus disease 19 (COVID-19). In individuals with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, vitamin D receptor activation is required to decrease acute respiratory distress syndrome. The purpose of this study was to examine the genotypic distribution and allelic frequencies of CDX2 rs11568820 and EcoRV rs4516035 polymorphisms in COVID-19 patients with various SARS-CoV-2 variants. For genotyping of CDX2 rs11568820 and EcoRV rs4516035 polymorphisms, we used the polymerase chain reaction-restriction fragment length polymorphism technique in 1734 and 1450 recovered and deceased patients, respectively. The results indicated the rate of COVID-19 mortality was associated with CDX2 rs11568820 AA and GA genotypes in the Delta variant and with CDX2 rs11568820 AA in the Omicron BA.5 variant, while no association was shown in the Alpha variant. Therefore, the rate of COVID-19 mortality was associated with EcoRV rs4516035 TC and CC genotypes in the Delta variant, while no association was shown in the Alpha and Omicron BA.5 variants. According to our analysis, the T-G haplotype was more common in all SARS-CoV-2 variants. The C-A haplotype was associated with COVID-19 mortality in the Delta and Omicron BA.5 variants, and the T-A haplotype was related to the Alpha variant. In conclusion, the genotype frequencies of the CDX2 rs11568820 and EcoRV rs4516035 polymorphisms between SARS-CoV-2 variants were significantly different between the deceased patients and recovered patients. However, more studies should be done to confirm the results.
多项研究表明，维生素 D 缺乏与患 2019 冠状病毒病（COVID-19）的风险增加有关。在严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）感染患者中，维生素 D 受体的激活可降低急性呼吸窘迫综合征的风险。本研究旨在探讨 CDX2 rs11568820 和 EcoRV rs4516035 多态性在不同 SARS-CoV-2 变异株 COVID-19 患者中的基因型分布和等位基因频率。我们使用聚合酶链反应-限制性片段长度多态性技术对 1734 名康复患者和 1450 名死亡患者的 CDX2 rs11568820 和 EcoRV rs4516035 多态性进行基因分型。结果表明，在 Delta 变异株中，COVID-19 死亡率与 CDX2 rs11568820 AA 和 GA 基因型有关，在 Omicron BA.5 变异株中与 CDX2 rs11568820 AA 有关，而在 Alpha 变异株中无关联。因此，在 Delta 变异株中，COVID-19 死亡率与 EcoRV rs4516035 TC 和 CC 基因型有关，而在 Alpha 和 Omicron BA.5 变异株中无关联。根据我们的分析，在所有 SARS-CoV-2 变异株中，T-G 单倍型更为常见。C-A 单倍型与 Delta 和 Omicron BA.5 变异株的 COVID-19 死亡率有关，而 T-A 单倍型与 Alpha 变异株有关。总之，CDX2 rs11568820 和 EcoRV rs4516035 多态性的基因型频率在 SARS-CoV-2 变异株之间在死亡患者和康复患者之间存在显著差异。然而，需要更多的研究来证实这些结果。
