Department of Laboratory Medicine, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, Taiwan.
Physical Examination Center, Show Chwan Memorial Hospital, Changhua 500, Taiwan.
Cells. 2023 Jun 15;12(12):1641. doi: 10.3390/cells12121641.
Serum and glucocorticoid-regulated kinase 1 (SGK1) is expressed in neuronal cells and involved in the pathogenesis of hypertension and metabolic syndrome, regulation of neuronal function, and depression in the brain. This study aims to identify the cellular mechanisms and signaling pathways of SGK1 in neuronal cells. In this study, the SGK1 inhibitor GSK650394 is used to suppress SGK1 expression in PC12 cells using an in vitro neuroscience research platform. Comparative transcriptomic analysis was performed to investigate the effects of SGK1 inhibition in nervous cells using mRNA sequencing (RNA-seq), differentially expressed genes (DEGs), and gene enrichment analysis. In total, 12,627 genes were identified, including 675 and 2152 DEGs at 48 and 72 h after treatment with GSK650394 in PC12 cells, respectively. Gene enrichment analysis data indicated that SGK1 inhibition-induced DEGs were enriched in 94 and 173 genes associated with vascular development and functional regulation and were validated using real-time PCR, Western blotting, and GEPIA2. Therefore, this study uses RNA-seq, DEG analysis, and GEPIA2 correlation analysis to identify positive candidate genes and signaling pathways regulated by SGK1 in rat nervous cells, which will enable further exploration of the underlying molecular signaling mechanisms of SGK1 and provide new insights into neuromodulation in cardiovascular diseases.
血清和糖皮质激素调节激酶 1（SGK1）在神经元细胞中表达，参与高血压和代谢综合征的发病机制、神经元功能的调节以及大脑中的抑郁。本研究旨在确定 SGK1 在神经元细胞中的细胞机制和信号通路。在这项研究中，使用 SGK1 抑制剂 GSK650394 在体外神经科学研究平台上抑制 PC12 细胞中的 SGK1 表达。使用 mRNA 测序（RNA-seq）进行比较转录组分析，以研究 SGK1 抑制对神经细胞的影响，包括差异表达基因（DEGs）和基因富集分析。总共鉴定出 12627 个基因，包括在 PC12 细胞中用 GSK650394 处理 48 和 72 小时后的 675 和 2152 个 DEGs。基因富集分析数据表明，SGK1 抑制诱导的 DEGs 富集了 94 和 173 个与血管发育和功能调节相关的基因，并通过实时 PCR、Western blot 和 GEPIA2 进行了验证。因此，本研究使用 RNA-seq、DEG 分析和 GEPIA2 相关性分析来鉴定大鼠神经细胞中由 SGK1 调节的阳性候选基因和信号通路，这将有助于进一步探索 SGK1 的潜在分子信号机制，并为心血管疾病中的神经调节提供新的见解。
