Ringens P J, Bistervels B, Hoenders H J, Wollensak J
Ophthalmic Res. 1986;18(2):61-7. doi: 10.1159/000265416.
Intumescent cataractous lenses were investigated as to their water content and protein composition. Nuclei were separated from cortices. Water-soluble, water-insoluble, urea-soluble and urea-insoluble portions were quantified and the subunit composition of the water-soluble crystallin fraction was looked at on SDS-gel electrophoresis. Compared to normal lenses it is observed that the water content in intumescent cataract is increased, water-soluble and urea-soluble fractions are diminished, whereas the urea-insoluble portions are augmented. No major changes are noted in the subunit composition of the soluble protein fractions. Particularly, the relative amount of gamma-crystallins is diminished in intumescent cataractous lenses.
对肿胀性白内障晶状体的含水量和蛋白质组成进行了研究。将晶状体核与皮质分离。对水溶性、水不溶性、尿素溶性和尿素不溶性部分进行了定量分析，并通过SDS凝胶电泳观察了水溶性晶状体蛋白部分的亚基组成。与正常晶状体相比，发现肿胀性白内障的含水量增加，水溶性和尿素溶性部分减少，而尿素不溶性部分增加。可溶性蛋白质部分的亚基组成没有明显变化。特别是，肿胀性白内障晶状体中γ-晶状体蛋白的相对含量减少。
