Yu Dingyi, Cui Shenghao, Chen Liqi, Zheng Shuang, Zhao Di, Yin Xinyu, Yang Faming, Chen Jingdi
Marine College, Shandong University, Weihai 264209, China.
Shandong Laboratory of Advanced Materials and Green Manufacturing, Yantai 265599, China.
Antioxidants (Basel). 2023 May 31;12(6):1190. doi: 10.3390/antiox12061190.
Peptide self-assembling materials have received significant attention from researchers in recent years, emerging as a popular field in biological, environmental, medical, and other new materials studies. In this study, we utilized controllable enzymatic hydrolysis technology (animal proteases) to obtain supramolecular peptide self-assembling materials (CAPs) from the Pacific oyster (). We conducted physicochemical analyses to explore the pro-healing mechanisms of CAPs on skin wounds in both in vitro and in vivo experiments through a topical application. The results demonstrated that CAPs exhibit a pH-responsive behavior for self-assembly and consist of peptides ranging from 550 to 2300 Da in molecular weight, with peptide chain lengths of mainly 11-16 amino acids. In vitro experiments indicated that CAPs display a procoagulant effect, free radical scavenging activity, and promote the proliferation of HaCaTs (112.74% and 127.61%). Moreover, our in vivo experiments demonstrated that CAPs possess the ability to mitigate inflammation, boost fibroblast proliferation, and promote revascularization, which accelerates the epithelialization process. Consequently, a balanced collagen I/III ratio in the repaired tissue and the promotion of hair follicle regeneration were observed. With these remarkable findings, CAPs can be regarded as a natural and secure treatment option with high efficacy for skin wound healing. The potential of CAPs to be further developed for traceless skin wound healing is an exciting area for future research and development.
近年来，肽自组装材料受到了研究人员的广泛关注，成为生物、环境、医学等新材料研究中的一个热门领域。在本研究中，我们利用可控酶解技术（动物蛋白酶）从太平洋牡蛎中获得了超分子肽自组装材料（CAPs）。我们进行了物理化学分析，通过局部应用在体外和体内实验中探索CAPs对皮肤伤口的促愈合机制。结果表明，CAPs表现出pH响应的自组装行为，由分子量在550至2300 Da之间的肽组成，肽链长度主要为11 - 16个氨基酸。体外实验表明，CAPs具有促凝血作用、自由基清除活性，并促进HaCaTs细胞增殖（分别为112.74%和127.61%）。此外，我们的体内实验表明，CAPs具有减轻炎症、促进成纤维细胞增殖和促进血管再生的能力，从而加速上皮化过程。因此，在修复组织中观察到I型/III型胶原蛋白比例平衡以及毛囊再生得到促进。基于这些显著发现，CAPs可被视为一种天然、安全且对皮肤伤口愈合具有高效性的治疗选择。CAPs在无痕皮肤伤口愈合方面进一步开发的潜力是未来研究与开发的一个令人兴奋的领域。
