Li Heng, Xu Liping, Wu Weiping, Peng Weizheng, Lou Yonggen, Lu Jing
State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Biology (Basel). 2023 Jun 5;12(6):820. doi: 10.3390/biology12060820.
It has been well documented that an infestation of the piercing-sucking herbivore, brown planthopper (BPH), , activates strong local defenses in rice. However, whether a BPH infestation elicits systemic responses in rice remains largely unknown. In this study, we investigated BPH-induced systemic defenses by detecting the change in expression levels of 12 JA- and/or SA-signaling-responsive marker genes in different rice tissues upon a BPH attack. We found that an infestation of gravid BPH females on rice leaf sheaths significantly increased the local transcript level of all 12 marker genes tested except , whose expression was induced only weakly at a later stage of the BPH infestation. Moreover, an infestation of gravid BPH females also systemically up-regulated the transcription levels of three JA-signaling-responsive genes (, , and ), one SA-signaling-responsive gene (), and two JA- and SA- signaling-responsive genes ( and ). Our results demonstrate that an infestation of gravid BPH females systemically activates JA- and SA-dependent defenses in rice, which may in turn influence the composition and structure of the community in the rice ecosystem.
有充分的文献记载，刺吸式植食性害虫褐飞虱（BPH）的侵害会激活水稻强烈的局部防御反应。然而，褐飞虱的侵害是否会引发水稻的系统反应在很大程度上仍不清楚。在本研究中，我们通过检测褐飞虱攻击后不同水稻组织中12个茉莉酸（JA）和/或水杨酸（SA）信号响应标记基因表达水平的变化，来研究褐飞虱诱导的系统防御。我们发现，孕性褐飞虱雌虫侵害水稻叶鞘后，除了 在褐飞虱侵害后期仅被微弱诱导表达外，所检测的12个标记基因的局部转录水平均显著增加。此外，孕性褐飞虱雌虫的侵害还系统性地上调了三个JA信号响应基因（ 、 和 ）、一个SA信号响应基因（ ）以及两个JA和SA信号响应基因（ 和 ）的转录水平。我们的结果表明，孕性褐飞虱雌虫的侵害会系统性地激活水稻中依赖JA和SA的防御反应，这反过来可能会影响水稻生态系统中群落的组成和结构。
