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一株假单胞菌的全线粒体基因组特征与系统进化分析。

Characterization and Phylogenetic Analysis of the Complete Mitochondrial Genome of .

机构信息

College of Life Science, Qufu Normal University, Qufu 273165, China.

出版信息

Genes (Basel). 2023 May 31;14(6):1205. doi: 10.3390/genes14061205.

Abstract

is a large diving duck belonging to the family Anatidae. However, the phylogenetic relationship among these species remains unclear due to the presence of extensive interspecific hybridization events within the genus. Here, we sequenced and annotated the complete mitochondrial genome of , which contained 22 tRNAs, 13 protein-coding genes (PCGs), 2 ribosomal RNAs, and 1 D-loop, with a length of 16,617 bp. The sizes of the PCGs ranged from 297 to 1824 bp and were all, except for , located on the heavy chain (H). ATG and TAA were the most common start and termination codons of the 13 PCGs, respectively. The fastest- and slowest-evolving genes were and , respectively. Codon usage analysis indicated that CUA, AUC, GCC, UUC, CUC, and ACC were the six most frequent codons. The nucleotide diversity values indicated a high level of genetic diversity in . analysis suggested a widespread gene exchange between and . Moreover, phylogenetic reconstructions using the mitochondrial genomes of all available Anatidae species showed that, in addition to , four major clades among the Anatidae (Dendrocygninae, Oxyurinae, Anserinae, and Anatinae) were closely related to . Overall, this study provides valuable information on the evolution of and new insights into the phylogeny of Anatidae.

摘要

绿头鸭是一种大型潜水鸭,属于鸭科。然而,由于该属内存在广泛的种间杂交事件,这些物种的系统发育关系仍不清楚。在这里,我们对绿头鸭的完整线粒体基因组进行了测序和注释,其长度为 16617bp,包含 22 个 tRNA、13 个蛋白编码基因(PCGs)、2 个核糖体 RNA 和 1 个 D 环。PCGs 的大小范围从 297 到 1824bp,除了 外,都位于重链(H)上。ATG 和 TAA 是 13 个 PCGs 最常见的起始和终止密码子。进化最快和最慢的基因分别是 和 。密码子使用分析表明,CUA、AUC、GCC、UUC、CUC 和 ACC 是六个最常见的密码子。核苷酸多样性值表明 具有很高的遗传多样性。 分析表明,和 之间存在广泛的基因交换。此外,使用所有可用鸭科物种的线粒体基因组进行的系统发育重建表明,除了 外,鸭科(秋沙鸭亚科、疣鼻栖鸭亚科、鸭亚科和雁亚科)的四个主要分支与 密切相关。总的来说,这项研究为 提供了有价值的进化信息,并为鸭科的系统发育提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7550/10298507/e95a53347ba0/genes-14-01205-g001.jpg

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