College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
Sichuan Provincial Engineering Research Center for Breeding Technology of Authentic Traditional Chinese Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Int J Mol Sci. 2023 Jun 17;24(12):10259. doi: 10.3390/ijms241210259.
Bunge is an important traditional herb. is distributed in the Sichuan province of China (here called SC). Under natural conditions, it does not bear seeds and its sterility mechanism is still unclear. Through artificial cross, there was defective pistil and partial pollen abortion in these plants. Electron microscopy results showed that the defective pollen wall was caused by delayed degradation of the tapetum. Due to the lack of starch and organelle, the abortive pollen grains showed shrinkage. RNA-seq was performed to explore the molecular mechanisms of pollen abortion. KEGG enrichment analysis suggested that the pathways of phytohormone, starch, lipid, pectin, and phenylpropanoid affected the fertility of . Moreover, some differentially expressed genes involved in starch synthesis and plant hormone signaling were identified. These results contribute to the molecular mechanism of pollen sterility and provide a more theoretical foundation for molecular-assisted breeding.
苍耳是一种重要的传统草药，分布在中国四川省（以下简称四川）。在自然条件下，它不结种子，其不育机制尚不清楚。通过人工杂交，这些植物的雌蕊存在缺陷，花粉部分败育。电镜结果表明，败育花粉壁是由于绒毡层降解延迟所致。由于缺乏淀粉和细胞器，败育花粉粒出现皱缩。通过 RNA-seq 技术来探索花粉败育的分子机制。KEGG 富集分析表明，植物激素、淀粉、脂质、果胶和苯丙烷途径影响苍耳的育性。此外，还鉴定到一些参与淀粉合成和植物激素信号转导的差异表达基因。这些结果有助于阐明花粉不育的分子机制，并为分子辅助育种提供更坚实的理论基础。
