Lockard R E, Currey K, Browner M, Lawrence C, Maizel J
Nucleic Acids Res. 1986 Jul 25;14(14):5827-41. doi: 10.1093/nar/14.14.5827.
A model for the secondary structure of mouse beta Maj globin messenger RNA is presented based on enzymatic digestion data, comparative sequence and computer analysis. Using 5'-32P-end-labeled beta globin mRNA as a substrate, single-stranded regions were determined with S1 and T1 nucleases and double-stranded regions with V1 ribonuclease from cobra venom. The structure data obtained for ca. 75% of the molecule was introduced into a computer algorithm which predicts secondary structures of minimum free energy consistent with the enzymatic data. Two prominent base paired regions independently derived by phylogenetic analysis were also present in the computer generated structure lending support for the model. An interesting feature of the model is the presence of long-range base pairing interactions which permit the beta globin mRNA to fold back on itself, thereby bringing the 5'- and 3'-noncoding regions within close proximity. This feature is consistent with data from other laboratories suggesting an interaction of the 5'- and 3'-domains in the mammalian globin mRNAs.
基于酶切数据、序列比较和计算机分析，提出了小鼠β-Maj珠蛋白信使核糖核酸二级结构的模型。以5'-32P末端标记的β-珠蛋白信使核糖核酸为底物，用S1和T1核酸酶确定单链区，用眼镜蛇毒的V1核糖核酸酶确定双链区。将约75%的分子获得的结构数据引入计算机算法，该算法预测与酶学数据一致的最小自由能二级结构。系统发育分析独立得出的两个突出的碱基配对区也存在于计算机生成的结构中，为该模型提供了支持。该模型的一个有趣特征是存在长程碱基配对相互作用，这使得β-珠蛋白信使核糖核酸能够自身折叠，从而使5'-和3'-非编码区紧密靠近。这一特征与其他实验室的数据一致，这些数据表明哺乳动物珠蛋白信使核糖核酸中5'-和3'-结构域之间存在相互作用。
