Topilina Yulia S, Luk'yanova Evgeniya A, Glukhova Lubov B, Shurupova Margarita N, Gerasimchuk Anna L, Frank Yulia A, Antsiferov Dmitry V
Biological Institute, National Research Tomsk State University, Lenina Ave., 36, 634050 Tomsk, Russia.
LLC Darwin, Str. Vysockogo Vladimira, 28, 634040 Tomsk, Russia.
Microorganisms. 2023 May 26;11(6):1406. doi: 10.3390/microorganisms11061406.
The paper searches for new solutions for the development of highbush blueberry orchards ( L. (1753)) in Western Siberia. All species of the genus display special symbiotic mycorrhizal associations with root systems-ericoid mycorrhiza, which essentially enhances the formation of adventitious and lateral roots. For the first time, we obtained pure cultures of micromycetes associated with the roots of wild species of the family in the Tomsk region, Russia. With regard to the data of molecular genetic analysis of the ITS region sequence, we selected the BR2-1 isolate based on its morphophysiological traits, which was assigned to the genus . Representatives of this genus typically enter into symbiotic relationships with heathers to form ericoid mycorrhizae. We studied the effect of strain BR2-1 on the development of microclones of the highbush blueberry var. Nord blue during their in vitro adaptation and showed its beneficial effect on growth and shoot formation in young plants. Experiments performed using submerged and solid-state methods showed that the most optimal method for commercial production of BR2-1 is cultivation on grain sterilized by boiling, followed by spore washing.
本文探寻了西西伯利亚高丛蓝莓果园（L. (1753)）发展的新解决方案。该属的所有物种都与根系形成特殊的共生菌根联合体——石楠状菌根，这从本质上促进了不定根和侧根的形成。我们首次在俄罗斯托木斯克地区获得了与该科野生物种根系相关的微真菌纯培养物。根据ITS区域序列的分子遗传分析数据，我们基于其形态生理特征选择了BR2-1分离株，它被归入该属。该属的代表通常与石南属植物建立共生关系以形成石楠状菌根。我们研究了BR2-1菌株对高丛蓝莓品种诺德蓝微克隆在离体适应过程中发育的影响，并表明其对幼苗生长和新梢形成具有有益作用。采用深层培养和固态培养方法进行的实验表明，BR2-1商业化生产的最优化方法是在煮沸灭菌的谷物上培养，随后进行孢子洗涤。
